HSP20 Antibody - #AF6003

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製品: HSP20 Antibody
カタログ: AF6003
タンパク質の説明: Rabbit polyclonal antibody to HSP20
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 18kDa; 17kD(Calculated).
ユニプロット: O14558
RRID: AB_2834938

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(85%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
HSP20 Antibody detects endogenous levels of total HSP20.
RRID:
AB_2834938
引用形式: Affinity Biosciences Cat# AF6003, RRID:AB_2834938.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

epididymis luminal protein 55; FLJ32389; Heat shock 20 kDa like protein p20; Heat shock 20 kDa-like protein p20; Heat shock protein alpha crystallin related B6; Heat shock protein beta 6; Heat shock protein beta-6; Heat shock protein, 20-KD; Heat-shock 27-KD protein 6; HEL55; Hsp20; HspB6; HSPB6_HUMAN;

免疫原

免疫原:
Uniprot:

O14558(HSPB6_HUMAN) >>Visit HPA database.

遺伝子(ID):
HSPB6(126393)
タンパク質の説明:
HSP20 a small heat shock protein of the HSP20 family. Note: This description may include information from UniProtKB.
タンパク質配列:
MEIPVPVQPSWLRRASAPLPGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
85
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14558 基板として

Site PTM Type Enzyme
S16 Phosphorylation Q15139 (PRKD1) , P17612 (PRKACA)
S59 Phosphorylation
S84 Phosphorylation

研究背景

機能:

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity.

PTMs:

The N-terminus is blocked.

Phosphorylated at Ser-16 by PKA and probably PKD1K; required to protect cardiomyocytes from apoptosis.

細胞の位置付け:

Cytoplasm. Nucleus. Secreted.
Note: Translocates to nuclear foci during heat shock.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer. Small heat shock proteins form high molecular mass oligomers containing variable number of monomers; these oligomers display a very flexible quaternary structure easily exchanging their subunits. Heterooligomer with HSPB1; formed through oligomerization of HSPB1:HSBP6 dimers; subunit exchange leads to formation of at least two different heterooligomeric complexes, differing in variable quantities of HSPB1 and HSPB6 homodimers in addition to HSPB1:HSPB6 heterodimers. Heterooligomer with CRYAB; large heterooligomers consist of CRYAB homodimers and HSPB5:HSPB6 heterodimers but lacking HSPB6 homodimers. Interacts with BAG3. Interacts (phosphorylated) with YWHAZ. Interacts with PDE4A and PDE4D; required for maintenance of the non-phosphorylated state of HSPB6 under basal conditions. Interacts with KDR. Interacts with PRKD1.

タンパク質ファミリー:

Belongs to the small heat shock protein (HSP20) family.

参考文献

1). Heat shock protein 20 suppresses breast carcinogenesis by inhibiting the MAPK and AKT signaling pathways. Oncology Letters, 2022 (PubMed: 36380873) [IF=2.9]

Application: WB    Species: Human    Sample: MDA-MB-468 cell

Figure 2. HSP20 overexpression inhibits BC cell proliferation. (A) Relative mRNA and protein expression levels of HSP20 in five BC cell lines compared with the MCF-10A normal mammary epithelial cell line. (B) Relative expression levels of HSP20 in MDA-MB-231 and MDA-MB-468 cells overexpressing HSP20. (C) Cell Counting Kit-8 proliferation assay. (D) Representative images and quantitative analysis of colony formation assays. Data are presented as the mean ± SD. **P<0.01; ***P<0.001 vs. MCF-10A or as indicated. BC, breast cancer; control, untransfected cells; vector, cells transfected with an empty vector; exHSP20, HSP20-overexpressing cells; HSP20, heat shock protein 20; OD, optical density.

Application: IHC    Species: Human    Sample: MDA-MB-468 cell

Figure 2. HSP20 overexpression inhibits BC cell proliferation. (A) Relative mRNA and protein expression levels of HSP20 in five BC cell lines compared with the MCF-10A normal mammary epithelial cell line. (B) Relative expression levels of HSP20 in MDA-MB-231 and MDA-MB-468 cells overexpressing HSP20. (C) Cell Counting Kit-8 proliferation assay. (D) Representative images and quantitative analysis of colony formation assays. Data are presented as the mean ± SD. **P<0.01; ***P<0.001 vs. MCF-10A or as indicated. BC, breast cancer; control, untransfected cells; vector, cells transfected with an empty vector; exHSP20, HSP20-overexpressing cells; HSP20, heat shock protein 20; OD, optical density.

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