製品: Kinesin 5A Antibody
カタログ: DF13467
タンパク質の説明: Rabbit polyclonal antibody to Kinesin 5A
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Rabbit, Dog
分子量: 117kDa, 160 kDa; 117kD(Calculated).
ユニプロット: Q12840
RRID: AB_2846486

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(88%), Sheep(100%), Rabbit(88%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Kinesin 5A Antibody detects endogenous levels of total Kinesin 5A.
RRID:
AB_2846486
引用形式: Affinity Biosciences Cat# DF13467, RRID:AB_2846486.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

D12S1889; KIF 5A; Kif5a; KIF5A_HUMAN; Kinesin family member 5A; Kinesin heavy chain isoform 5A; Kinesin Heavy Chain Neuron Specific; Kinesin heavy chain neuron-specific 1; MY050; Neuronal kinesin heavy chain; NKHC 1; NKHC; SPG 10;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q12840 KIF5A_HUMAN:

Distributed throughout the CNS but is highly enriched in subsets of neurons.

タンパク質配列:
MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVVIGGKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEEIRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQALEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSEFSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADEVKKALELQMESHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLYERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGATSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Horse
88
Rabbit
88
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12840 基板として

Site PTM Type Enzyme
A2 Acetylation
T152 Phosphorylation
S155 Phosphorylation
K160 Ubiquitination
T170 Phosphorylation
S205 Phosphorylation
K227 Ubiquitination
K241 Ubiquitination
K253 Ubiquitination
T298 Phosphorylation
T299 Phosphorylation
S316 Phosphorylation
T317 Phosphorylation
T326 Phosphorylation
K348 Acetylation
S705 Phosphorylation
T806 Phosphorylation
T807 Phosphorylation
S831 Phosphorylation
K842 Ubiquitination
K845 Ubiquitination
K883 Acetylation
K888 Acetylation
K890 Acetylation
R899 Methylation
S931 Phosphorylation

研究背景

機能:

Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

細胞の位置付け:

Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Perikaryon.
Note: Concentrated in the cell body of the neurons, particularly in the perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Distributed throughout the CNS but is highly enriched in subsets of neurons.

サブユニット構造:

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with FMR1 (via C-terminus); this interaction is increased in a mGluR-dependent manner. Interacts with ZFYVE27. Interacts with VAPA, VAPB, SURF4, RAB11A (GDP-bound form), RAB11B (GDP-bound form) and RTN3 in a ZFYVE27-dependent manner (By similarity). Interacts with BORCS5. Interacts with BICD2.

タンパク質ファミリー:

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

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