Lysyl tRNA synthetase Antibody - #DF13475
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13475, RRID:AB_2846494.
折りたたみ／展開
CMTRIB; DFNB89; EC 6.1.1.6; KARS 1; KARS 2; KARS; KARS1; KARS2; KIAA0070; KRS; Lysine tRNA ligase; Lysine--tRNA ligase; LysRS; Lysyl tRNA synthetase; Lysyl-tRNA synthetase; SYK_HUMAN;
免疫原
- Q15046 SYK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15046 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| K78 | Ubiquitination | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Methylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| R148 | Methylation | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K175 | Acetylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| K249 | Ubiquitination | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| R314 | Methylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| T366 | Phosphorylation | Uniprot | |
| K402 | Acetylation | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot |
研究背景
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.
(Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Phosphorylated on a serine residue after mast cell stimulation with immunoglobulin E (IgE).
Cytoplasm>Cytosol. Cytoplasm. Nucleus. Cell membrane>Peripheral membrane protein. Secreted.
Note: Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739).
Mitochondrion.
Homodimer and tetradimer. Part of the multisynthetase complex (MSC), a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with AIMP2 (via N-terminus) and MITF. Interacts with TARSL2.
(Microbial infection) Interacts directly with HIV-1 virus GAG protein.
The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain, is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
Belongs to the class-II aminoacyl-tRNA synthetase family.
研究領域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
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