AK2 Antibody - #DF13478
| 製品: | AK2 Antibody |
| カタログ: | DF13478 |
| タンパク質の説明: | Rabbit polyclonal antibody to AK2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 26kDa; 26kD(Calculated). |
| ユニプロット: | P54819 |
| RRID: | AB_2846497 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13478, RRID:AB_2846497.
折りたたみ／展開
Adenylate kinase 2; adenylate kinase 2, mitochondrial; Adenylate kinase isoenzyme 2; ADK2; AK 2; ak2; ATP AMP transphosphorylase; ATP-AMP transphosphorylase 2; EC 2.7.4.3; KAD2_HUMAN; mitochondrial;
免疫原
Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
- P54819 KAD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPSVPAAEPEYPKGIRAVLLGPPGAGKGTQAPRLAENFCVCHLATGDMLRAMVASGSELGKKLKATMDAGKLVSDEMVVELIEKNLETPLCKNGFLLDGFPRTVRQAEMLDDLMEKRKEKLDSVIEFSIPDSLLIRRITGRLIHPKSGRSYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKIRLQAYHTQTTPLIEYYRKRGIHSAIDASQTPDVVFASILAAFSKATCKDLVMFI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P54819 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| Y12 | Phosphorylation | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| C40 | S-Nitrosylation | Uniprot | |
| C42 | S-Nitrosylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K72 | Acetylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| C92 | S-Nitrosylation | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| Y201 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| T215 | Phosphorylation | Uniprot | |
| C232 | S-Nitrosylation | Uniprot | |
| K233 | Ubiquitination | Uniprot |
研究背景
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
Mitochondrion intermembrane space.
Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
Monomer.
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.
Belongs to the adenylate kinase family. AK2 subfamily.
研究領域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Thiamine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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