C6orf134 Antibody - #DF13487
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(4)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13487, RRID:AB_2846506.
折りたたみ／展開
Acetyltransferase mec 17 homolog; Acetyltransferase mec-17 homolog; Alpha TAT; Alpha tubulin acetyltransferase 1; Alpha tubulin N acetyltransferase; Alpha-TAT; Alpha-tubulin N-acetyltransferase; ATAT_HUMAN; ATAT1; Chromosome 6 open reading frame 134; Hypothetical protein LOC79969; MEC17; Nbla00487; TAT;
免疫原
- Q5SQI0 ATAT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTRGTPPGLVAQSCCYSRHGGVNSSSPNTGNQDSKQGEQETKNRSASEEQALSQDGSGEKPMHTAPPQAPAPPAQSWTVGGDILNARFIRNLQERRSTRPW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q5SQI0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K56 | Methylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K233 | Acetylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| R293 | Methylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| T322 | Phosphorylation | Uniprot | |
| R323 | Methylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| K380 | Acetylation | Uniprot |
研究背景
Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.
Autoacetylation strongly increases tubulin acetylation.
Cytoplasm. Membrane>Clathrin-coated pit. Cell junction>Focal adhesion. Cell projection>Axon. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle.
Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.
Belongs to the acetyltransferase ATAT1 family.
参考文献
Application: WB Species: Rat Sample:
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