MTR Antibody - #DF13489

(1)   (2)  
製品: MTR Antibody
カタログ: DF13489
タンパク質の説明: Rabbit polyclonal antibody to MTR
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 140kDa; 141kD(Calculated).
ユニプロット: Q99707
RRID: AB_2846508

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
MTR Antibody detects endogenous levels of total MTR.
RRID:
AB_2846508
引用形式: Affinity Biosciences Cat# DF13489, RRID:AB_2846508.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

5-methyltetrahydrofolate homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase; cblG; Methionine synthase; methioninesynthase; MS; MTR; MTR1; PRP20; SRM1; Vitamin-B12 dependent methionine synthase;

免疫原

免疫原:
Uniprot:

Q99707(METH_HUMAN) >>Visit HPA database.

遺伝子(ID):
MTR(4548)
発現特異性:
Q99707 METH_HUMAN:

Widely expressed. Expressed at the highest levels in pancreas, heart, brain, skeletal muscle and placenta. Expressed at lower levels in lung, liver and kidney.

タンパク質配列:
MSPALQDLSQPEGLKKTLRDEINAILQKRIMVLDGGMGTMIQREKLNEEHFRGQEFKDHARPLKGNNDILSITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQNLFEEKYAPRPIFISGTIVDKSGRTLSGQTGEGFVISVSHGEPLCIGLNCALGAAEMRPFIEIIGKCTTAYVLCYPNAGLPNTFGDYDETPSMMAKHLKDFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKPRVPPATAFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALCVAKVQVEMGAQVLDVNMDDGMLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKYGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNAGNLPVYDDIHKELLQLCEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKGIEKHIIEDTEEARLNQKKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKWLGPILGYDTD

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Zebrafish
88
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99707 基板として

Site PTM Type Enzyme
S2 Phosphorylation
K16 Ubiquitination
T17 Phosphorylation
K28 Ubiquitination
T39 Phosphorylation
K45 Ubiquitination
K57 Ubiquitination
K64 Ubiquitination
S121 Phosphorylation
K127 Ubiquitination
T133 Phosphorylation
K139 Ubiquitination
K151 Ubiquitination
K231 Ubiquitination
K309 Ubiquitination
K339 Ubiquitination
K388 Ubiquitination
Y395 Phosphorylation
K466 Ubiquitination
K475 Ubiquitination
K484 Ubiquitination
Y491 Phosphorylation
Y520 Phosphorylation
K565 Ubiquitination
K638 Ubiquitination
K645 Ubiquitination
K658 Ubiquitination
K659 Ubiquitination
Y677 Phosphorylation
K681 Ubiquitination
K685 Ubiquitination
K699 Ubiquitination
K700 Ubiquitination
Y701 Phosphorylation
K727 Ubiquitination
K735 Ubiquitination
K742 Ubiquitination
K753 Ubiquitination
T758 Phosphorylation
T764 Phosphorylation
T774 Phosphorylation
K781 Ubiquitination
K789 Ubiquitination
K814 Ubiquitination
K817 Methylation
K817 Ubiquitination
K823 Ubiquitination
K865 Ubiquitination
K871 Ubiquitination
S877 Phosphorylation
Y905 Phosphorylation
Y913 Phosphorylation
Y921 Phosphorylation
K925 Ubiquitination
Y929 Phosphorylation
K952 Ubiquitination
K995 Ubiquitination
K1000 Ubiquitination
K1008 Ubiquitination
Y1010 Phosphorylation
S1022 Phosphorylation
K1024 Ubiquitination
K1071 Ubiquitination
K1127 Ubiquitination
R1132 Methylation
S1155 Phosphorylation
K1169 Ubiquitination
K1186 Ubiquitination
S1206 Phosphorylation
Y1219 Phosphorylation
S1221 Phosphorylation
K1226 Ubiquitination
K1232 Ubiquitination
K1235 Ubiquitination
K1245 Ubiquitination

研究背景

機能:

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity).

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Expressed at the highest levels in pancreas, heart, brain, skeletal muscle and placenta. Expressed at lower levels in lung, liver and kidney.

サブユニット構造:

Monomer. Dimer. Mainly monomer. Interacts with MTRR.

タンパク質ファミリー:

Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin (By similarity).

Belongs to the vitamin-B12 dependent methionine synthase family.

研究領域

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

参考文献

1). DNA methyltransferases-associated long non-coding RNA PRKCQ-AS1 regulate DNA methylation in myelodysplastic syndrome. International journal of laboratory hematology, 2024 (PubMed: 38679027) [IF=3.0]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.