CPEB4 Antibody - #DF13495
| 製品: | CPEB4 Antibody |
| カタログ: | DF13495 |
| タンパク質の説明: | Rabbit polyclonal antibody to CPEB4 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 80kDa; 80kD(Calculated). |
| ユニプロット: | Q17RY0 |
| RRID: | AB_2846514 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13495, RRID:AB_2846514.
折りたたみ／展開
CPE binding protein 4; CPE-binding protein 4; CPE-BP4; cpeb4; CPEB4_HUMAN; Cytoplasmic polyadenylation element binding protein 4; Cytoplasmic polyadenylation element-binding protein 4; hCPEB-4; KIAA1673;
免疫原
Expressed in pancreas in islets and ductal cells (at protein level) (PubMed:22138752). Expressed in melanocytes (PubMed:27857118).
- Q17RY0 CPEB4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDYGFGVLVQSNTGNKSAFPVRFHPHLQPPHHHQNATPSPAAFINNNTAANGSSAGSAWLFPAPATHNIQDEILGSEKAKSQQQEQQDPLEKQQLSPSPGQEAGILPETEKAKSEENQGDNSSENGNGKEKIRIESPVLTGFDYQEATGLGTSTQPLTSSASSLTGFSNWSAAIAPSSSTIINEDASFFHQGGVPAASANNGALLFQNFPHHVSPGFGGSFSPQIGPLSQHHPHHPHFQHHHSQHQQQRRSPASPHPPPFTHRNAAFNQLPHLANNLNKPPSPWSSYQSPSPTPSSSWSPGGGGYGGWGGSQGRDHRRGLNGGITPLNSISPLKKNFASNHIQLQKYARPSSAFAPKSWMEDSLNRADNIFPFPDRPRTFDMHSLESSLIDIMRAENDTIKGRLNYSYPGSDSSLLINARTYGRRRGQSSLFPMEDGFLDDGRGDQPLHSGLGSPHCFSHQNGERVERYSRKVFVGGLPPDIDEDEITASFRRFGPLIVDWPHKAESKSYFPPKGYAFLLFQDESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHGEIDKRVEVKPYVLDDQLCDECQGARCGGKFAPFFCANVTCLQYYCEYCWAAIHSRAGREFHKPLVKEGGDRPRHISFRWN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q17RY0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R23 | Methylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| R251 | Methylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| K505 | Acetylation | Uniprot | |
| K509 | Acetylation | Uniprot | |
| K554 | Ubiquitination | Uniprot | |
| Y604 | Phosphorylation | Uniprot | |
| Y609 | Phosphorylation | Uniprot | |
| T614 | Phosphorylation | Uniprot | |
| Y620 | Phosphorylation | Uniprot | |
| Y635 | Phosphorylation | Uniprot | |
| S725 | Phosphorylation | Uniprot |
研究背景
Sequence-specific RNA-binding protein that binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism. Regulates activation of unfolded protein response (UPR) in the process of adaptation to ER stress in liver, by maintaining translation of CPE-regulated mRNAs in conditions in which global protein synthesis is inhibited (By similarity). Required for cell cycle progression, specifically for cytokinesis and chromosomal segregation. Plays a role as an oncogene promoting tumor growth and progression by positively regulating translation of t-plasminogen activator/PLAT. Stimulates proliferation of melanocytes. In contrast to CPEB1 and CPEB3, does not play role in synaptic plasticity, learning and memory (By similarity).
Cytoplasm. Cell projection>Dendrite. Cell projection>Dendritic spine. Cell junction>Synapse>Postsynaptic density. Cell projection>Axon. Cell projection>Growth cone. Endoplasmic reticulum. Cytoplasm>Perinuclear region.
Expressed in pancreas in islets and ductal cells (at protein level). Expressed in melanocytes.
Interacts with TOB1.
The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA (PubMed:24990967). The interdomain linker (564-579) acts as a hinge to fix the relative orientation of the 2 RRMs (PubMed:24990967). The ZZ domain (509-566) coordinates 2 Zn ions and is probably implicated in mediating interactions with other proteins in addition to increasing the affinity of the RRMs for the CPEs (By similarity). Unlike in CPEB1, a continuous polar interface is formed between the 2 RRMs (PubMed:24990967).
Belongs to the RRM CPEB family.
研究領域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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