RASGRF2 Antibody - #DF13513
| 製品: | RASGRF2 Antibody |
| カタログ: | DF13513 |
| タンパク質の説明: | Rabbit polyclonal antibody to RASGRF2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Dog |
| 分子量: | 140kDa; 141kD(Calculated). |
| ユニプロット: | O14827 |
| RRID: | AB_2846532 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13513, RRID:AB_2846532.
折りたたみ／展開
GRF2; Ras guanine nucleotide exchange factor 2; Ras protein specific guanine nucleotide releasing factor 2; Ras-GRF2; Ras-specific guanine nucleotide-releasing factor 2; RASGRF2; RGRF2_HUMAN;
免疫原
Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
- O14827 RGRF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKTAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEGCSCERTPAPPRAGAGQGGVRDALDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPDAAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKAAANILRALSQDDQDDIHLKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVAQYLLDKDLIIDEDTLYELSLKIEPRLPA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14827 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S37 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| K207 | Acetylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| T630 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S737 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| T748 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| T855 | Phosphorylation | Uniprot | |
| S1000 | Phosphorylation | Uniprot | |
| S1002 | Phosphorylation | Uniprot | |
| Y1090 | Phosphorylation | Uniprot | |
| S1098 | Phosphorylation | Uniprot | |
| T1111 | Phosphorylation | Uniprot |
研究背景
Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
Phosphorylated by CDK5; down-regulates RASGRF2-mediated RAC1 activation.
Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome (By similarity).
Cytoplasm. Cell membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein.
Note: Translocates to membranes when activated. Found both at cell periphery and along the axon of neurons (By similarity).
Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
Homooligomer and heterooligomer with RASGRF1. Interacts with Ras and RAC1. Interacts in a calcium-dependent manner with calmodulin (By similarity). Interacts with CDK5R1 and EPB49. Interacts with the AMPA receptor through GRIA1 (By similarity). Interacts with microtubules (By similarity).
The Ras-GEF domain and the N-terminal Ras-GEF domain form a Ras-binding site and mediate Ras activation.
The IQ domain mediates the calcium-dependent interaction with calmodulin but is dispensable for the Ras-GEF activity.
The DH (DBL-homology) domain mediates interaction with RASGRF1 and EPB49 and is required for RAC1 activation.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
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