BIN1 Antibody - #DF13520
| 製品: | BIN1 Antibody |
| カタログ: | DF13520 |
| タンパク質の説明: | Rabbit polyclonal antibody to BIN1 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Horse, Rabbit, Dog |
| 分子量: | 64kDa; 65kD(Calculated). |
| ユニプロット: | O00499 |
| RRID: | AB_2846539 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13520, RRID:AB_2846539.
折りたたみ／展開
AMPH 2; AMPH2; Amphiphysin 2; Amphiphysin II; Amphiphysin like protein; amphiphysin-like; Amphiphysin-like protein; AMPHL; Bin1; BIN1_HUMAN; Box Dependant MYC Interacting Protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; DKFZp547F068; MGC10367; MGC105358; Myc box dependent interacting protein 1; Myc box-dependent-interacting protein 1; SH3P9;
免疫原
Ubiquitous. Highest expression in the brain and muscle (PubMed:9182667). Expressed in oligodendrocytes (PubMed:27488240). Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240). Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
- O00499 BIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLASVKAMHEASKKLNECLQEVYEPDWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQWCQGKLQAHLVAQTNLLRNQAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQFEAPGPFSEQASLLDLDFDPLPPVTSPVKAPTPSGQSIPWDLWEPTESPAGSLPSGEPSAAEGTFAVSWPSQTAEPGPAQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00499 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| R66 | Methylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T348 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| T534 | Phosphorylation | Uniprot |
研究背景
Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production. In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation. Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro.
Phosphorylated by protein kinase C.
Nucleus. Cytoplasm. Endosome. Cell membrane>Sarcolemma>T-tubule.
Cytoplasm.
Ubiquitous. Highest expression in the brain and muscle. Expressed in oligodendrocytes. Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Heterodimer with AMPH (By similarity). Binds SH3GLB1 (By similarity). Interacts (via SH3 domain) with DNM1. Interacts with SYNJ1 (By similarity). Interacts (via SH3 domain) with DNM2. Isoform IIA interacts with CLTC. Isoform IIB does not interact with CLTC. Isoform IIC1 does not interact with CLTC. Isoform IIC2 does not interact with CLTC. Interacts with AP2A2. Interacts with AP2B1 (By similarity). Interacts with MYC (via N-terminal transactivation domain); the interaction requires the integrity of the conserved MYC box regions 1 and 2 (By similarity). Interacts with BIN2. Interacts with SNX4. Interacts (via BAR domain) with BACE1. Binds (via BAR domain) F-actin.
(Microbial infection) Interacts (SH3 domain) with HCV NS5A.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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