AMH Antibody - #DF13527

(2)   (2)  
製品: AMH Antibody
カタログ: DF13527
タンパク質の説明: Rabbit polyclonal antibody to AMH
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Dog
分子量: 59kDa; 59kD(Calculated).
ユニプロット: P03971
RRID: AB_2846546

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
AMH Antibody detects endogenous levels of total AMH.
RRID:
AB_2846546
引用形式: Affinity Biosciences Cat# DF13527, RRID:AB_2846546.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Anti muellerian hormone; MIF; MIS; Muellerian inhibiting factor; Mullerian inhibiting substance;

免疫原

免疫原:

A synthesized peptide derived from human AMH, corresponding to a region within C-terminal amino acids.

Uniprot:

P03971(MIS_HUMAN) >>Visit HPA database.

遺伝子(ID):
AMH(268)
タンパク質配列:
MRDLPLTSLALVLSALGALLGTEALRAEEPAVGTSGLIFREDLDWPPGSPQEPLCLVALGGDSNGSSSPLRVVGALSAYEQAFLGAVQRARWGPRDLATFGVCNTGDRQAALPSLRRLGAWLRDPGGQRLVVLHLEEVTWEPTPSLRFQEPPPGGAGPPELALLVLYPGPGPEVTVTRAGLPGAQSLCPSRDTRYLVLAVDRPAGAWRGSGLALTLQPRGEDSRLSTARLQALLFGDDHRCFTRMTPALLLLPRSEPAPLPAHGQLDTVPFPPPRPSAELEESPPSADPFLETLTRLVRALRVPPARASAPRLALDPDALAGFPQGLVNLSDPAALERLLDGEEPLLLLLRPTAATTGDPAPLHDPTSAPWATALARRVAAELQAAAAELRSLPGLPPATAPLLARLLALCPGGPGGLGDPLRALLLLKALQGLRVEWRGRDPRGPGRAQRSAGATAADGPCALRELSVDLRAERSVLIPETYQANNCQGVCGWPQSDRNPRYGNHVVLLLKMQVRGAALARPPCCVPTAYAGKLLISLSEERISAHHVPNMVATECGCR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the TGF-beta family.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

参考文献

1). Anti-Müllerian hormone regulates ovarian granulosa cell growth in PCOS rats through SMAD4. International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics, 2025 (PubMed: 39865361) [IF=2.6]
2). AMH regulates ovarian granulosa cell growth in PCOS rats through SMAD4. Research Square, 2024

Application: WB    Species: Rat    Sample:

Figure 1. Expression of AMH and SMAD4 in serum, ovarian tissue and granulosa cells of PCOS rats 1A Vaginal smears (Jimsa staining 200×); (A-D) control (E-H)DHEA group; A pre-motility (B,E-H) motility C late motility D inter-motility a nucleated epithelial cells bnon-nucleated keratinised cells c leukocytes. 1B HE staining (40×) of rat ovaries; (A-C) 10, 15, 20 days oil control group; (D-F) 10, 15, 20 days DHEA group;(G-I) 10, 15, 20 days blank control group: primary follicle (PF), secondary follicle (SF), mature follicle (MF), sinus follicle (An), atretic follicle (At), corpus luteum (CL). 1C On days 10, 15, and 20 of modeling, T, AMH, and SMAD4 levels in rat serum were measured by ELISA. 1D Localisation of AMH and SMAD4 proteins in rat ovarian tissues (400×); (A,D) Oil control group (B,E)PCOS group (C,F) Blank control group; White arrows show positive expression of granulosa cells: a granulosa cells b follicular membrane cells. 1E Western blotting to detect the expression levels of AMH and SMAD4 in blank control group, oil control group and DHEA group((NC)20-day blank control group (C) oil control group (U) DHEA group). 1F Identification of rat ovarian granulosa cells: (A,B) HE staining of cells; (C-E)FSHR immunofluorescence; A 200×; B 400×; CDAPI; D FSHR; E MERGE; white arrowheads show cell nucleus. 1G (A-E)Western blotting to detect the expression of AMH, SMAD4, PCNA, BAX in granulosa cells of PCOS group and control group. F mRNA levels of SMAD4 in granulosa cells of the oil control and PCOS groups were detected by RT-qPCR. All data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.