Histone H1.5 Antibody - #DF13539
(1)
(14)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13539, RRID:AB_2846558.
折りたたみ／展開
H1; H1 histone family member 5; H1.5; H15 HUMAN; H15_HUMAN; H1B; H1F5; H1s 3; Hist1h1b; Histone 1 H1b; Histone cluster 1 H1b; Histone H1.5; Histone H1a; Histone H1b; Histone H1s 3; MGC126630; MGC126632;
免疫原
- P16401 H15_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSETAPAETATPAPVEKSPAKKKATKKAAGAGAAKRKATGPPVSELITKAVAASKERNGLSLAALKKALAAGGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGATPKKAKKAAGAKKAVKKTPKKAKKPAAAGVKKVAKSPKKAKAAAKPKKATKSPAKPKAVKPKAAKPKAAKPKAAKPKAAKAKKAAAKKK
PTMs - P16401 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | P49841 (GSK3B) , PR:P49841 (hGSK3B) | Uniprot |
| K17 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K27 | Methylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K37 | Methylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| K49 | Acetylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| Y74 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K132 | Acetylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K133 | Acetylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| K140 | Acetylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| K149 | Acetylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K168 | Acetylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K209 | Acetylation | Uniprot |
研究背景
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter (By similarity). Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase.
Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Nucleus. Chromosome.
Note: According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.
Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
The C-terminal domain is required for high-affinity binding to chromatin.
Belongs to the histone H1/H5 family.
参考文献
Application: WB Species: Mice Sample: OSRC2 cells
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