GLUT5 Antibody - #DF13545

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製品: GLUT5 Antibody
カタログ: DF13545
タンパク質の説明: Rabbit polyclonal antibody to GLUT5
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Dog
分子量: 50~70kDa; 55kD(Calculated).
ユニプロット: P22732
RRID: AB_2846564

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(88%), Bovine(88%), Horse(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Glucose Transporter 5 GLUT5 Antibody detects endogenous levels of total Glucose Transporter 5 GLUT5.
RRID:
AB_2846564
引用形式: Affinity Biosciences Cat# DF13545, RRID:AB_2846564.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Facilitated glucose transporter member 5; Fructose transporter; Glucose transporte, kidney; Glucose Transporter 5 GLUT5; glucose transporter like protein 5; Glucose transporter type 5; Glucose transporter type 5 small intestine; GLUT 5; GLUT-5; GLUT5; GTR5_HUMAN; SLC 2A5; SLC2A5; small intestine; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; Solute carrier family 2;

免疫原

免疫原:
Uniprot:

P22732(GTR5_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC2A5(6518)
発現特異性:
P22732 GTR5_HUMAN:

Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level) (PubMed:7619085). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

タンパク質配列:
MEQQDQSMKEGRLTLVLALATLIAAFGSSFQYGYNVAAVNSPALLMQQFYNETYYGRTGEFMEDFPLTLLWSVTVSMFPFGGFIGSLLVGPLVNKFGRKGALLFNNIFSIVPAILMGCSRVATSFELIIISRLLVGICAGVSSNVVPMYLGELAPKNLRGALGVVPQLFITVGILVAQIFGLRNLLANVDGWPILLGLTGVPAALQLLLLPFFPESPRYLLIQKKDEAAAKKALQTLRGWDSVDREVAEIRQEDEAEKAAGFISVLKLFRMRSLRWQLLSIIVLMGGQQLSGVNAIYYYADQIYLSAGVPEEHVQYVTAGTGAVNVVMTFCAVFVVELLGRRLLLLLGFSICLIACCVLTAALALQDTVSWMPYISIVCVISYVIGHALGPSPIPALLITEIFLQSSRPSAFMVGGSVHWLSNFTVGLIFPFIQEGLGPYSFIVFAVICLLTTIYIFLIVPETKAKTFIEINQIFTKMNKVSEVYPEKEELKELPPVTSEQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
88
Bovine
88
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22732 基板として

Site PTM Type Enzyme
M1 Acetylation
K9 Ubiquitination
R120 Methylation
T199 Phosphorylation
K477 Ubiquitination
K488 Ubiquitination
K492 Ubiquitination

研究背景

機能:

Functions as a fructose transporter that has only low activity with other monosaccharides. Can mediate the uptake of 2-deoxyglucose, but with low efficiency. Essential for fructose uptake in the small intestine (By similarity). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose (By similarity). Required for the development of high blood pressure in response to high dietary fructose intake (By similarity).

細胞の位置付け:

Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cell membrane>Sarcolemma.
Note: Localized on the apical membrane of jejunum villi, but also on lateral plasma membranes of the villi. Transport to the cell membrane is dependent on RAB11A.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

タンパク質ファミリー:

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

研究領域

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

参考文献

1). Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice. JCI Insight, 2022 (PubMed: 35077394) [IF=8.0]

Application: WB    Species: Mice    Sample: Hippocampal tissues

Figure 5 RH reduced brain GLUT3-mediated glucose uptake in STZ-induced APP/PS1-DM mice. (A) Representative PET/CT images showing in vivo brain 18F-FDG uptake (coronal, sagittal, and transaxial sections, n = 6 mice for each group). Cor, Cortex; Hip, hippocampus; Mid, midbrain; HPOA, hypothalamus; BS, brain stem; Cer, cerebellum; OB, Olfactory Bulb; BF, basal forebrain; Tha, thalamus; SC, superior colliculi; CG, central gray; Str, striatum. (B) Quantification for SUV of 18F-FDG in brain region (n = 6 for mice for each group). (C) Western blot and quantitation for GLUT1, GLUT2, GLUT3, GLUT4, GLUT5, SGLT1, and SGLT2 in hippocampal homogenates (n = 3 mice for each group). The data are expressed as the mean ± SEM. Statistical significance was assessed using unpaired Student’s t test. *P < 0.05 and **P < 0.01, APP/PS1-DM versus APP/PS1; #P < 0.05 and ##P < 0.01, APP/PS1-DM-RH versus APP/PS1-DM; WT versus APP/PS1; NS, no significant difference.
2). The mTORC1/eIF4E/HIF-1α pathway mediates glycolysis to support brain hypoxia resistance in the Gansu zokor, Eospalax cansus. Frontiers in Physiology, 2021 (PubMed: 33708138) [IF=4.0]

Application: WB    Species: Rat    Sample: brain tissues

FIGURE 4 Translation level analysis of glycolysis-related transporters and enzymes in Sprague-Dawley rat and Gansu zokor. GLUT1 glucose transporter (A), PFK (B), GLUT5 glucose transporter (C), and KHK (D) protein expression. Representative images of at least three separate blots below each bar graph showing expression of the proteins. All data are presented as mean ± SEM. Statistical symbols: #P < 0.05 and ##P < 0.01 between two species under the same oxygen treatment; *P < 0.05 and **P < 0.01 between different oxygen treatments in the same species.

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