SERBP1 Antibody - #DF13553

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製品: SERBP1 Antibody
カタログ: DF13553
タンパク質の説明: Rabbit polyclonal antibody to SERBP1
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 44kDa; 45kD(Calculated).
ユニプロット: Q8NC51
RRID: AB_2846572

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
SERBP1 Antibody detects endogenous levels of total SERBP1.
RRID:
AB_2846572
引用形式: Affinity Biosciences Cat# DF13553, RRID:AB_2846572.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CGI 55; CHD3IP; Chromodomain helicase DNA binding protein 3 interacting protein; DKFZp564M2423; FLJ90489; HABP4L; PAI 1 mRNA binding protein; PAI RBP1; PAI-RBP1; PAI1 RNA-binding protein 1; PAIRB_HUMAN; PAIRBP1; Plasminogen activator inhibitor 1 RNA binding protein; Plasminogen activator inhibitor 1 RNA-binding protein; Serbp1; SERPINE1 mRNA binding protein 1; SERPINE1 mRNA-binding protein 1;

免疫原

免疫原:
Uniprot:

Q8NC51(PAIRB_HUMAN) >>Visit HPA database.

遺伝子(ID):
SERBP1(26135)
発現特異性:
Q8NC51 PAIRB_HUMAN:

Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain.

タンパク質配列:
MPGHLQEGFGCVVTNRFDQLFDDESDPFEVLKAAENKKKEAGGGGVGGPGAKSAAQAAAQTNSNAAGKQLRKESQKDRKNPLPPSVGVVDKKEETQPPVALKKEGIRRVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NC51 基板として

Site PTM Type Enzyme
C11 S-Nitrosylation
S25 Phosphorylation
K32 Ubiquitination
K39 Ubiquitination
K52 Sumoylation
K52 Ubiquitination
S53 Phosphorylation
T61 Phosphorylation
S63 Phosphorylation
K68 Acetylation
K68 Ubiquitination
S85 Phosphorylation
K92 Ubiquitination
T95 Phosphorylation
K102 Acetylation
K102 Sumoylation
K102 Ubiquitination
K122 Acetylation
K122 Ubiquitination
R126 Methylation
K140 Acetylation
K140 Ubiquitination
S152 Phosphorylation
R155 Methylation
R160 Methylation
R163 Methylation
R165 Methylation
R177 Methylation
R181 Methylation
R188 Methylation
R195 Methylation
S197 Phosphorylation
R201 Methylation
S202 Phosphorylation
S203 Phosphorylation
S205 Phosphorylation
Y207 Phosphorylation
S208 Phosphorylation
K211 Acetylation
K211 Ubiquitination
R216 Methylation
S219 Phosphorylation
S221 Phosphorylation
K228 Sumoylation
K228 Ubiquitination
T232 Phosphorylation
S234 Phosphorylation
K236 Ubiquitination
Y237 Phosphorylation
K240 Ubiquitination
S243 Phosphorylation
Y244 Phosphorylation
Y246 Phosphorylation
S247 Phosphorylation
S252 Phosphorylation
T255 Phosphorylation
T258 Phosphorylation
K281 Sumoylation
K281 Ubiquitination
K286 Ubiquitination
K294 Ubiquitination
K303 Ubiquitination
K310 Ubiquitination
K320 Ubiquitination
K321 Acetylation
K321 Ubiquitination
K327 Methylation
K327 Ubiquitination
S328 Phosphorylation
S330 Phosphorylation
S338 Phosphorylation
K346 Ubiquitination
S353 Phosphorylation
R364 Methylation
R367 Methylation
R370 Methylation
R373 Methylation
T388 Phosphorylation
K390 Ubiquitination
S391 Phosphorylation
S392 Phosphorylation
S394 Phosphorylation

研究背景

機能:

May play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay. Seems to play a role in PML-nuclear bodies formation.

細胞の位置付け:

Cytoplasm. Nucleus. Cytoplasm>Perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain.

サブユニット構造:

Interacts with SPIN1 (By similarity). Interacts with CHD3 and TDRD3. Interacts with ZDHHC17 (via ANK repeats).

参考文献

1). Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling. Molecular Medicine Reports, 2020 (PubMed: 33174043) [IF=3.4]

Application: WB    Species: Mice    Sample: HEI-OC1 cells

Figure 5. FPKM values and expression of several DEGs in the three groups. (A-D) FPKM values of several random selected up- or downregulated DEGs with typical expression profiling in response to treatment with cisplatin alone or combined with Tet. (E) Expression of several protein products of DEGs. *P<0.05, vs. control. #P<0.05 vs. Cisplatin. The differences were analyzed by one-way ANOVA followed by Tukey's post hoc test. DEG, differentially expressed gene; FKPM, fragments per kilobase of exon model per million reads mapped.
2). Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy. Free radical research, 2023 (PubMed: 37897414) [IF=3.3]
3). TGF-β1-Mediated Activation of SERPINE1 is Involved in Hemin-Induced Apoptotic and Inflammatory Injury in HT22 Cells. Neuropsychiatric Disease and Treatment, 2021 (PubMed: 33603380) [IF=3.2]

Application: WB    Species: Mice    Sample: HT22 cells

Figure 1 SERPINE1 is upregulated in hemin-treated HT22 cells. (A) HT22 cells were treated with different doses (0, 10, 20, 40, 60, 80, 100, and 120 μM) of hemin for 24 h and then subjected to MTT assay. (B) HT22 cells were treated with 100 μM of hemin for 24 h and then assayed for SERPINE1 expression using qRT-PCR assay. (C) Representative image of Western blot analysis and quantification of SERPINE1 expression in HT22 cells stimulated with hemin. *p < 0.05, **p <0.01, and ***p <0.001.

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