Synaptojanin Antibody - #DF13566
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13566, RRID:AB_2846585.
折りたたみ／展開
4; 5-trisphosphate 5-phosphatase 1; Inositol 5' phosphatase (synaptojanin 1); Inositol 5' phosphatase; INPP 5G; INPP5G; KIAA0910; PARK20; Polyphosphoinositide phosphatase; Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 1; Synaptic inositol 145 trisphosphate 5 phosphatase 1; Synaptic inositol-1; Synaptojanin 1; Synaptojanin 1 polyphosphoinositide phosphatase; Synaptojanin-1; Synaptojanin1; Synj 1; Synj1; SYNJ1_HUMAN;
免疫原
Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.
- O43426 SYNJ1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYSKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRIDSSDEDRISEVRKVLNSGNFYFAWSASGISLDLSLNAHRSMQEQTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVVYLDDSVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAPAFDRHFRTLKNLYGKQIIVNLLGSKEGEHMLSKAFQSHLKASEHAADIQMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFNGSEVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTRFQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKARALLTTGSLRVSEQTLQSASSKVLKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVSASTTNQKLWAVELQKTISRDNKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKERNEDFIEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQVFRGFLEGKVTFAPTYKYDLFSDDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYTWTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQNIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQQFASFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEEMSLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGPPSAQSSPIDAQPATPLPQKDPAQPLEPKRPPPPRPVAPPTRPAPPQRPPPPSGARSPAPTRKEFGGIGAPPSPGVARREMEAPKSPGTTRKDNIGRSQPSPQAGLAGPGPAGYSTARPTIPPRAGVISAPQSHARASAGRLTPESQSKTSETSKGSTFLPEPLKPQAAFPPQSSLPPPAQRLQEPLVPVAAPMPQSGPQPNLETPPQPPPRSRSSHSLPSEASSQPQVKTNGISDGKRESPLKIDPFEDLSFNLLAVSKAQLSVQTSPVPTPDPKRLIQLPSATQSNVLSSVSCMPTMPPIPARSQSQENMRSSPNPFITGLTRTNPFSDRTAAPGNPFRAKSEESEATSWFSKEEPVTISPFPSLQPLGHNKSRASSSLDGFKDSFDLQGQSTLKISNPKGWVTFEEEEDFGVKGKSKSACSDLLGNQPSSFSGSNLTLNDDWNKGTNVSFCVLPSRRPPPPPVPLLPPGTSPPVDPFTTLASKASPTLDFTER
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43426 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| R284 | Methylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| K352 | Ubiquitination | Uniprot | |
| T443 | Phosphorylation | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| T498 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| K515 | Acetylation | Uniprot | |
| K518 | Acetylation | Uniprot | |
| K554 | Methylation | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| T680 | Phosphorylation | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| K798 | Ubiquitination | Uniprot | |
| S830 | Phosphorylation | Uniprot | |
| S1044 | Phosphorylation | Uniprot | |
| S1045 | Phosphorylation | Uniprot | |
| T1048 | Phosphorylation | Uniprot | |
| S1049 | Phosphorylation | Uniprot | |
| S1053 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | Uniprot | |
| S1083 | Phosphorylation | Uniprot | |
| T1092 | Phosphorylation | Uniprot | |
| S1150 | Phosphorylation | Uniprot | |
| T1166 | Phosphorylation | Uniprot | |
| S1178 | Phosphorylation | Uniprot | |
| Y1191 | Phosphorylation | Uniprot | |
| T1220 | Phosphorylation | Uniprot | |
| S1223 | Phosphorylation | Uniprot | |
| T1230 | Phosphorylation | Uniprot | |
| T1282 | Phosphorylation | Uniprot | |
| S1290 | Phosphorylation | Uniprot | |
| S1292 | Phosphorylation | Uniprot | |
| S1293 | Phosphorylation | Uniprot | |
| S1295 | Phosphorylation | Uniprot | |
| S1302 | Phosphorylation | Uniprot | |
| S1318 | Phosphorylation | Uniprot | |
| S1341 | Phosphorylation | Uniprot | |
| S1345 | Phosphorylation | Uniprot | |
| T1349 | Phosphorylation | Uniprot | |
| S1385 | Phosphorylation | Uniprot | |
| S1391 | Phosphorylation | Uniprot | |
| S1392 | Phosphorylation | Uniprot | |
| R1409 | Methylation | Uniprot | |
| S1421 | Phosphorylation | Uniprot | |
| S1439 | Phosphorylation | Uniprot | |
| S1456 | Phosphorylation | Uniprot | |
| S1457 | Phosphorylation | Uniprot | |
| S1529 | Phosphorylation | Uniprot | |
| S1551 | Phosphorylation | Uniprot | |
| S1565 | Phosphorylation | Uniprot |
研究背景
Phosphatase that acts on various phosphoinositides, including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol (3,4,5)-trisphosphate. Has a role in clathrin-mediated endocytosis (By similarity). Hydrolyzes PIP2 bound to actin regulatory proteins resulting in the rearrangement of actin filaments downstream of tyrosine kinase and ASH/GRB2 (By similarity).
Cytoplasm>Perinuclear region.
Concentrated at clathrin-coated endocytic intermediates in nerve terminals. Isoform 1 is more enriched than isoform 2 in developing brain as well as non-neuronal cells. Isoform 2 is very abundant in nerve terminals.
Interacts with ASH/GRB2. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Binds AMPH, SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). Interacts with BIN1 and DNM1 (By similarity).
Binds to EPS15 (a clathrin coat-associated protein) via a C-terminal domain containing three Asn-Pro-Phe (NPF) repeats.
The C-terminal proline-rich region mediates binding to a variety of SH3 domain-containing proteins including AMPH, SH3GL1, SH3GL2, SH3GL3 and GRB2.
Belongs to the synaptojanin family.
In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.
研究領域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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