KMT5C/SUV4-20h2 Antibody - #DF13570
| 製品: | KMT5C/SUV4-20h2 Antibody |
| カタログ: | DF13570 |
| タンパク質の説明: | Rabbit polyclonal antibody to KMT5C/SUV4-20h2 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog |
| 分子量: | 52kDa,60kDa; 52kD(Calculated). |
| ユニプロット: | Q86Y97 |
| RRID: | AB_2846589 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13570, RRID:AB_2846589.
折りたたみ／展開
FLJ98627; Histone lysine N methyltransferase SUV420H2; Histone-lysine N-methyltransferase SUV420H2; KMT5C; Lysine N methyltransferase 5C; Lysine N-methyltransferase 5C; MGC2705; Su(var)4 20 homolog 2; Su(var)4-20 homolog 2; Suppressor of variegation 4 20 homolog 2; Suppressor of variegation 4-20 homolog 2; Suv4 20h2; Suv4-20h2; SUV420H2; SV422_HUMAN;
免疫原
- Q86Y97 KMT5C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPDRVTARELCENDDLATSLVLDPYLGFRTHKMNVSPVPPLRRQQHLRSALETFLRQRDLEAAYRALTLGGWTARYFQSRGPRQEAALKTHVYRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVGCIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAACVKVLRDIEPGDEVTCFYGEGFFGEKNEHCECHTCERKGEGAFRTRPREPALPPRPLDKYQLRETKRRLQQGLDSGSRQGLLGPRACVHPSPLRRDPFCAACQPLRLPACSARPDTSPLWLQWLPQPQPRVRPRKRRRPRPRRAPVLSTHHAARVSLHRWGGCGPHCRLRGEALVALGQPPHARWAPQQDWHWARRYGLPYVVRVDLRRLAPAPPATPAPAGTPGPILIPKQALAFAPFSPPKRLRLVVSHGSIDLDVGGEEL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86Y97 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T69 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| Y259 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot |
研究背景
Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
Nucleus. Chromosome.
Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).
Homodimer (By similarity). Interacts with HP1 proteins CBX1, CBX3 and CBX5. Interacts with RB1 family proteins RB1, RBL1 and RBL2 (By similarity).
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
研究領域
· Metabolism > Amino acid metabolism > Lysine degradation.
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