VPS29 Antibody - #DF13575
製品: | VPS29 Antibody |
カタログ: | DF13575 |
タンパク質の説明: | Rabbit polyclonal antibody to VPS29 |
アプリケーション: | WB IF/ICC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 20kDa; 21kD(Calculated). |
ユニプロット: | Q9UBQ0 |
RRID: | AB_2846594 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13575, RRID:AB_2846594.
折りたたみ/展開
DC15; DC7; DKFZp564F0223; FLJ20492; hVPS29; PEP11; PEP11 homolog; Retromer protein; Vacuolar protein sorting 29; Vacuolar protein sorting 29 (yeast homolog); Vacuolar protein sorting 29 (yeast); Vacuolar protein sorting 29 homolog (S. cerevisiae); Vacuolar protein sorting 29 homolog; Vacuolar protein sorting associated protein 29; Vacuolar protein sorting-associated protein 29; Vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; VPS 29; VPS29; VPS29 retromer complex component; VPS29_HUMAN; x 007 protein;
免疫原
Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
- Q9UBQ0 VPS29_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKFEAFEHENKFYINPGSATGAYNALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UBQ0 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S17 | Phosphorylation | Uniprot | |
K24 | Ubiquitination | Uniprot | |
K30 | Ubiquitination | Uniprot | |
K43 | Acetylation | Uniprot | |
K43 | Ubiquitination | Uniprot | |
K50 | Acetylation | Uniprot | |
K50 | Ubiquitination | Uniprot | |
Y69 | Phosphorylation | Uniprot | |
K73 | Acetylation | Uniprot | |
K73 | Sumoylation | Uniprot | |
K73 | Ubiquitination | Uniprot | |
K173 | Sumoylation | Uniprot | |
K173 | Ubiquitination | Uniprot |
研究背景
Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Acts also as component of the retriever complex. The retriever complex is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). In the endosomes, retriever complex drives the retrieval and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling. The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with ANKRD27.
(Microbial infection) The heterotrimeric retromer cargo-selective complex (CSC) mediates the exit of human papillomavirus from the early endosome and the delivery to the Golgi apparatus.
Cytoplasm. Membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein. Early endosome. Late endosome.
Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
Component of the heterotrimeric retromer cargo-selective complex (CSC), also described as vacuolar protein sorting subcomplex (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35. The CSC has a highly elongated structure with VPS26 and VPS29 binding independently at opposite distal ends of VPS35 as central platform (By similarity). The CSC is believed to associate with variable sorting nexins to form functionally distinct retromer complex variants. The originally described retromer complex (also called SNX-BAR retromer) is a pentamer containing the CSC and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to form the SNX27-retromer complex. Component of the heterotrimeric retriever complex formed by VPS26C, VPS29 and VPS35L. Interacts with VPS26A, VPS35, SNX1, SNX2, SNX27, WASHC5, TBC1D5. Interacts with VPS26B and ANKRD27 (By similarity).
(Microbial infection) Interacts with human papillomavirus 16 minor capsid protein L2 (via C-terminus); this interaction mediates the transport of the capsid from the early endosome to the Golgi apparatus.
Belongs to the VPS29 family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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