DHCR7 Antibody - #DF13577

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製品: DHCR7 Antibody
カタログ: DF13577
タンパク質の説明: Rabbit polyclonal antibody to DHCR7
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 54kDa; 54kD(Calculated).
ユニプロット: Q9UBM7
RRID: AB_2846596

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
DHCR7 Antibody detects endogenous levels of total DHCR7.
RRID:
AB_2846596
引用形式: Affinity Biosciences Cat# DF13577, RRID:AB_2846596.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

7 dehydrocholesterol reductase; 7 DHC reductase; Delta 7 dehydrocholesterol reductase; Putative sterol reductase SR 2; SLOS; Sterol delta 7 reductase; Sterol Delta;

免疫原

免疫原:
Uniprot:

Q9UBM7(DHCR7_HUMAN) >>Visit HPA database.

遺伝子(ID):
DHCR7(1717)
発現特異性:
Q9UBM7 DHCR7_HUMAN:

Widely expressed. Most abundant in adrenal gland, liver, testis, and brain.

タンパク質配列:
MAAKSQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLYTLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQAWLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFPTSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELHSHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
88
Chicken
88
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBM7 基板として

Site PTM Type Enzyme
K4 Ubiquitination
S5 Phosphorylation
K13 Ubiquitination
S14 Phosphorylation
T19 Phosphorylation
S25 Phosphorylation
K88 Ubiquitination
T135 Phosphorylation
S205 Phosphorylation
K231 Ubiquitination
K358 Ubiquitination
K376 Ubiquitination
C380 S-Nitrosylation
Y382 Phosphorylation
S397 Phosphorylation
K454 Ubiquitination

研究背景

機能:

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Most abundant in adrenal gland, liver, testis, and brain.

サブユニット構造:

Interacts with DHCR24; this interaction regulates DHCR7 activity.

タンパク質ファミリー:

Belongs to the ERG4/ERG24 family.

研究領域

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

参考文献

1). Augmentation of 3β-hydroxysteroid-Δ24 Reductase (DHCR24) Expression Induced by Bovine Viral Diarrhea Virus Infection Facilitates Viral Replication via Promoting Cholesterol Synthesis. Journal of Virology, 2022 (PubMed: 36468862) [IF=5.4]

Application: WB    Species: bovine    Sample: bovine cells

FIG 1 (A) Cholesterol synthesis pathway. (B) Heat map of the key genes (transcriptomic data) and corresponding proteins (proteomic data) involved in lipid metabolism in bovine viral diarrhea virus (BVDV)-infected bovine cells. (C, D) Identification of differentially expressed genes by quantitative reverse transcription-PCR (qRT-PCR) (C) and corresponding proteins by Western blotting (D). HMGCS1, 3- hydroxy-3-methylglutaryl-coenzyme A synthase 1; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; MVK, mevalonate kinase; PMVK, phosphomevalonate kinase; MVD, diphosphomevalonate decarboxylase; FDPS, farnesyl diphosphate synthase; FDFT1, farnesyl diphosphate farnesyltransferase 1; SQLE, squalene epoxidase; GGPS, geranylgeranyl pyrophosphate synthase; LSS, lanosterol synthase; D8D7I, 3β-hydroxysterol-Δ8,7-isomerase; C5SD, 3β-hydroxysterol-C5 desaturase; DHCR7, 7-dehydrocholesterol reductase; DHCR24, 3β-hydroxysteroid-Δ24 reductase; SCD1, stearoyl-CoA desaturase; FASN, fatty acid synthase; ACACA, acetyl coenzyme A carboxylase alpha.

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