SSX2IP Antibody - #DF13582
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13582, RRID:AB_2846601.
折りたたみ／展開
ADIP; ADIP_HUMAN; Afadin and alpha actinin binding protein; Afadin DIL domain interacting protein; Afadin DIL domain-interacting protein; Afadin- and alpha-actinin-binding protein; FLJ10848; KIAA0923; MGC75026; OTTHUMP00000011574; SSX 2 interacting protein; SSX2 interacting protein; SSX2-interacting protein; SSX2IP; Synovial sarcoma X breakpoint 2 interacting protein;
免疫原
Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.
- Q9Y2D8 ADIP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDWMTVTDPGLSSESKTISQYTSETKMSPSSLYSQQVLCSSIPLSKNVHSFFSAFCTEDNIEQSISYLDQELTTFGFPSLYEESKGKETKRELNIVAVLNCMNELLVLQRKNLLAQENVETQNLKLGSDMDHLQSCYSKLKEQLETSRREMIGLQERDRQLQCKNRNLHQLLKNEKDEVQKLQNIIASRATQYNHDMKRKEREYNKLKERLHQLVMNKKDKKIAMDILNYVGRADGKRGSWRTGKTEARNEDEMYKILLNDYEYRQKQILMENAELKKVLQQMKKEMISLLSPQKKKPRERVDDSTGTVISDVEEDAGELSRESMWDLSCETVREQLTNSIRKQWRILKSHVEKLDNQVSKVHLEGFNDEDVISRQDHEQETEKLELEIQQCKEMIKTQQQLLQQQLATAYDDDTTSLLRDCYLLEEKERLKEEWSLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWLKQQFLNMTTFDHQNSENVKLFSAFSGSSDWDNLIVHSRQPQKKPHSVSNGSPVCMSKLTKSLPASPSTSDFCQTRSCISEHSSINVLNITAEEIKPNQVGGECTNQKWSVASRPGSQEGCYSGCSLSYTNSHVEKDDLP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y2D8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T18 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| T454 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| K582 | Acetylation | Uniprot | |
| S591 | Phosphorylation | Uniprot |
研究背景
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.
Cell junction>Adherens junction. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Not found at cell-matrix AJs.
Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.
Interacts with afadin and alpha-actinin (By similarity). Interacts with VAV2 (By similarity). Interacts with SSX2 and SSX3. Does not interact with SSX1 and SSX4. Interacts with PCM1 (By similarity). Interacts with WRAP73.
Both the N-terminal (up to position 79) and the C-terminal (from position 304) sequences are required for interaction with SSX2.
Belongs to the ADIP family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
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