Anillin Antibody - #DF13590
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13590, RRID:AB_2846609.
折りたたみ/展開
Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Actin-binding protein anillin; Anillin actin binding protein; anln; ANLN_HUMAN; DKFZp779A055; FSGS8; Scra; Scraps; Scraps homolog;
免疫原
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus (PubMed:24676636). Expression is significantly increased in renal biopsy specimens from idiopathic FSGS (PubMed:24676636). Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
- Q9NQW6 ANLN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPFTEKLLERTRARRENLQRKMAERPTAAPRSMTHAKRARQPLSEASNQQPLSGGEEKSCTKPSPSKKRCSDNTEVEVSNLENKQPVESTSAKSCSPSPVSPQVQPQAADTISDSVAVPASLLGMRRGLNSRLEATAASSVKTRMQKLAEQRRRWDNDDMTDDIPESSLFSPMPSEEKAASPPRPLLSNASATPVGRRGRLANLAATICSWEDDVNHSFAKQNSVQEQPGTACLSKFSSASGASARINSSSVKQEATFCSQRDGDASLNKALSSSADDASLVNASISSSVKATSPVKSTTSITDAKSCEGQNPELLPKTPISPLKTGVSKPIVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERCQEHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQKELACLRGRFDKGNIWSAEKGGNSKSKQLETKQETHCQSTPLKKHQGVSKTQSLPVTEKVTENQIPAKNSSTEPKGFTECEMTKSSPLKITLFLEEDKSLKVTSDPKVEQKIEVIREIEMSVDDDDINSSKVINDLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQTVGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVPRAESGDSLGSEDRDLLYSIDAYRSQRFKETERPSIKQVIVRKEDVTSKLDEKNNAFPCQVNIKQKMQELNNEINMQQTVIYQASQALNCCVDEEHGKGSLEEAEAERLLLIATGKRTLLIDELNKLKNEGPQRKNKASPQSEFMPSKGSVTLSEIRLPLKADFVCSTVQKPDAANYYYLIILKAGAENMVATPLASTSNSLNGDALTFTTTFTLQDVSNDFEINIEVYSLVQKKDPSGLDKKKKTSKSKAITPKRLLTSITTKSNIHSSVMASPGGLSAVRTSNFALVGSYTLSLSSVGNTKFVLDKVPFLSSLEGHIYLKIKCQVNSSVEERGFLTIFEDVSGFGAWHRRWCVLSGNCISYWTYPDDEKRKNPIGRINLANCTSRQIEPANREFCARRNTFELITVRPQREDDRETLVSQCRDTLCVTKNWLSADTKEERDLWMQKLNQVLVDIRLWQPDACYKPIGKP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NQW6 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
S54 | Phosphorylation | Uniprot | |
S65 | Phosphorylation | Uniprot | |
S67 | Phosphorylation | Uniprot | |
C71 | S-Nitrosylation | Uniprot | |
S72 | Phosphorylation | Uniprot | |
T75 | Phosphorylation | Uniprot | |
K85 | Ubiquitination | Uniprot | |
S92 | Phosphorylation | Uniprot | |
S95 | Phosphorylation | Uniprot | |
S97 | Phosphorylation | Uniprot | |
S99 | Phosphorylation | Uniprot | |
S102 | Phosphorylation | Uniprot | |
T112 | Phosphorylation | Uniprot | |
S140 | Phosphorylation | Uniprot | |
S141 | Phosphorylation | Uniprot | |
K143 | Ubiquitination | Uniprot | |
S168 | Phosphorylation | Uniprot | |
S169 | Phosphorylation | Uniprot | |
S172 | Phosphorylation | Uniprot | |
S176 | Phosphorylation | Uniprot | |
S182 | Phosphorylation | Uniprot | |
S189 | Phosphorylation | Uniprot | |
T194 | Phosphorylation | Uniprot | |
S211 | Phosphorylation | Uniprot | |
S219 | Phosphorylation | Uniprot | |
S225 | Phosphorylation | Uniprot | |
S236 | Phosphorylation | Uniprot | |
K237 | Ubiquitination | Uniprot | |
S245 | Phosphorylation | Uniprot | |
S250 | Phosphorylation | Uniprot | |
S252 | Phosphorylation | Uniprot | |
K254 | Sumoylation | Uniprot | |
K254 | Ubiquitination | Uniprot | |
S261 | Phosphorylation | Uniprot | |
S268 | Phosphorylation | Uniprot | |
K271 | Ubiquitination | Uniprot | |
S274 | Phosphorylation | Uniprot | |
S276 | Phosphorylation | Uniprot | |
S281 | Phosphorylation | Uniprot | |
S289 | Phosphorylation | Uniprot | |
T294 | Phosphorylation | Uniprot | |
S295 | Phosphorylation | Uniprot | |
S299 | Phosphorylation | Uniprot | |
T300 | Phosphorylation | Uniprot | |
K307 | Ubiquitination | Uniprot | |
S308 | Phosphorylation | Uniprot | |
C309 | S-Nitrosylation | Uniprot | |
T320 | Phosphorylation | Uniprot | |
S323 | Phosphorylation | Uniprot | |
T327 | Phosphorylation | Uniprot | |
S330 | Phosphorylation | Uniprot | |
T337 | Phosphorylation | Uniprot | |
S339 | Phosphorylation | Uniprot | |
T341 | Phosphorylation | Uniprot | |
S344 | Phosphorylation | Uniprot | |
K345 | Ubiquitination | Uniprot | |
S349 | Phosphorylation | Uniprot | |
S356 | Phosphorylation | Uniprot | |
S358 | Phosphorylation | Uniprot | |
K359 | Ubiquitination | Uniprot | |
S362 | Phosphorylation | Uniprot | |
T363 | Phosphorylation | Uniprot | |
T364 | Phosphorylation | Uniprot | |
T368 | Phosphorylation | Uniprot | |
K371 | Acetylation | Uniprot | |
K371 | Ubiquitination | Uniprot | |
S388 | Phosphorylation | Uniprot | |
S392 | Phosphorylation | Uniprot | |
T393 | Phosphorylation | Uniprot | |
T397 | Phosphorylation | Uniprot | |
T401 | Phosphorylation | Uniprot | |
T404 | Phosphorylation | Uniprot | |
K413 | Sumoylation | Uniprot | |
K413 | Ubiquitination | Uniprot | |
T416 | Phosphorylation | Uniprot | |
S417 | Phosphorylation | Uniprot | |
S418 | Phosphorylation | Uniprot | |
S419 | Phosphorylation | Uniprot | |
T421 | Phosphorylation | Uniprot | |
K428 | Methylation | Uniprot | |
K428 | Sumoylation | Uniprot | |
K428 | Ubiquitination | Uniprot | |
K433 | Ubiquitination | Uniprot | |
S449 | Phosphorylation | Uniprot | |
K452 | Ubiquitination | Uniprot | |
K464 | Sumoylation | Uniprot | |
S471 | Phosphorylation | Uniprot | |
T472 | Phosphorylation | Uniprot | |
K482 | Ubiquitination | Uniprot | |
T483 | Phosphorylation | Uniprot | |
S485 | Phosphorylation | Uniprot | |
K500 | Ubiquitination | Uniprot | |
T504 | Phosphorylation | Uniprot | |
T515 | Phosphorylation | Uniprot | |
S517 | Phosphorylation | Uniprot | |
S518 | Phosphorylation | Uniprot | |
T523 | Phosphorylation | Uniprot | |
K530 | Ubiquitination | Uniprot | |
S531 | Phosphorylation | Uniprot | |
K533 | Ubiquitination | Uniprot | |
S536 | Phosphorylation | Uniprot | |
K543 | Ubiquitination | Uniprot | |
S553 | Phosphorylation | Uniprot | |
S561 | Phosphorylation | Uniprot | |
S562 | Phosphorylation | Uniprot | |
S570 | Phosphorylation | Uniprot | |
S621 | Phosphorylation | Uniprot | |
S624 | Phosphorylation | Uniprot | |
T628 | Phosphorylation | Uniprot | |
K634 | Ubiquitination | Uniprot | |
S637 | Phosphorylation | Uniprot | |
S639 | Phosphorylation | Uniprot | |
S642 | Phosphorylation | Uniprot | |
K647 | Ubiquitination | Uniprot | |
S658 | Phosphorylation | Uniprot | |
S661 | Phosphorylation | Uniprot | |
S664 | Phosphorylation | Uniprot | |
Y671 | Phosphorylation | Uniprot | |
S672 | Phosphorylation | Uniprot | |
Y676 | Phosphorylation | Uniprot | |
S678 | Phosphorylation | Uniprot | |
K682 | Ubiquitination | Uniprot | |
S688 | Phosphorylation | Uniprot | |
K690 | Ubiquitination | Uniprot | |
K702 | Ubiquitination | Uniprot | |
K706 | Ubiquitination | Uniprot | |
K717 | Ubiquitination | Uniprot | |
K719 | Ubiquitination | Uniprot | |
T767 | Phosphorylation | Uniprot | |
K769 | Ubiquitination | Uniprot | |
T771 | Phosphorylation | Uniprot | |
K779 | Ubiquitination | Uniprot | |
K790 | Ubiquitination | Uniprot | |
S792 | Phosphorylation | Uniprot | |
S795 | Phosphorylation | Uniprot | |
K801 | Ubiquitination | Uniprot | |
S913 | Phosphorylation | Uniprot | |
S918 | Phosphorylation | Uniprot | |
S927 | Phosphorylation | Uniprot | |
K956 | Acetylation | Uniprot | |
T1079 | Phosphorylation | Uniprot | |
K1092 | Sumoylation | Uniprot | |
K1092 | Ubiquitination | Uniprot | |
K1101 | Ubiquitination | Uniprot | |
Y1118 | Phosphorylation | Uniprot |
研究背景
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration.
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1.
Nucleus. Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Cell projection>Bleb.
Note: Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus. Expression is significantly increased in renal biopsy specimens from idiopathic FSGS. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.
参考文献
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.