Surfactant protein D Antibody - #DF13601

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製品: Surfactant protein D Antibody
カタログ: DF13601
タンパク質の説明: Rabbit polyclonal antibody to Surfactant protein D
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 38kDa; 38kD(Calculated).
ユニプロット: P35247
RRID: AB_2846620

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Surfactant protein D Antibody detects endogenous levels of total Surfactant protein D.
RRID:
AB_2846620
引用形式: Affinity Biosciences Cat# DF13601, RRID:AB_2846620.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

COLEC 7; COLEC7; Collectin-7; Collectin7; Lung surfactant protein D; PSP D; PSP-D; PSP-D Surfactant protein D; PSPD; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein D; Pulmonary surfactant associated protein PSP-D; Pulmonary surfactant-associated protein D; SFTP 4; SFTP4; SFTPD; SFTPD_HUMAN; SP D; SP-D; Surfactant associated protein pulmonary 4; Surfactant protein D; Surfactant pulmonary associated protein D;

免疫原

免疫原:
Uniprot:

P35247(SFTPD_HUMAN) >>Visit HPA database.

遺伝子(ID):
SFTPD(6441)
発現特異性:
P35247 SFTPD_HUMAN:

Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).

タンパク質配列:
MLLFLLSALVLLTQPLGYLEAEMKTYSHRTMPSACTLVMCSSVESGLPGRDGRDGREGPRGEKGDPGLPGAAGQAGMPGQAGPVGPKGDNGSVGEPGPKGDTGPSGPPGPPGVPGPAGREGPLGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSAGARGLAGPKGERGVPGERGVPGNTGAAGSAGAMGPQGSPGARGPPGLKGDKGIPGDKGAKGESGLPDVASLRQQVEALQGQVQHLQAAFSQYKKVELFPNGQSVGEKIFKTAGFVKPFTEAQLLCTQAGGQLASPRSAAENAALQQLVVAKNEAAFLSMTDSKTEGKFTYPTGESLVYSNWAPGEPNDDGGSEDCVEIFTNGKWNDRACGEKRLVVCEF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
86
Pig
71
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35247 基板として

Site PTM Type Enzyme
T30 Phosphorylation
S33 Phosphorylation
C35 S-Nitrosylation
C40 S-Nitrosylation

研究背景

機能:

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

PTMs:

The N-terminus is blocked.

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix. Secreted>Extracellular space>Surface film.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).

サブユニット構造:

Oligomeric complex of 4 set of homotrimers.

タンパク質ファミリー:

Belongs to the SFTPD family.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

参考文献

1). GTS-21 attenuates ACE/ACE2 ratio and glycocalyx shedding in lipopolysaccharide-induced acute lung injury by targeting macrophage polarization derived ADAM-17. International immunopharmacology, 2024 (PubMed: 38310766) [IF=5.6]

Application: WB    Species: Mouse    Sample: lung tissue

Fig. 2. GTS-21 regulated the ACE/ACE2 ratio and glycocalyx shedding in LPS-ALI mice A-D ELISA kits were used to quantify the concentrations of major renin-angiotensin system (RAS) regulating enzymes (ACE, Ang II, ACE2, and Ang 1-7) in the lung tissue (n = 6 per group). E Protein levels of important RAS-regulating enzymes (ACE, AT1R, ACE2, and MasR) (n = 3 per group). The relative protein levels are displayed. F Levels of NF-κB phosphoproteins (p-P65 and p-IκB) (n = 3 per group). The relative protein levels are displayed. The immunofluorescence of HS, SDC-1, and surfactant protein D in the lungs is shown in G in representative images and densitometry (scar bar, 20 mm, n = 3 per group). H Evans Blue staining tested lung permeability (n = 3 for each group). Using ELISA, we assessed the BALF concentrations of HS I and HPA J; SP-D in serum K and BALF L (n = 5 for each group). M Relative protein levels of SDC-1, HPA, EXT-1, and SP-D in The lung tissues (n = 3 for each group). All data were shown as mean ± SD.
2). Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats. JOURNAL OF ETHNOPHARMACOLOGY, 2023 (PubMed: 36535334) [IF=5.4]
3). PC (16:0/14:0) ameliorates hyperoxia-induced bronchopulmonary dysplasia by upregulating claudin-1 and promoting alveolar type II cell repair. The international journal of biochemistry & cell biology, 2024 (PubMed: 38740281) [IF=4.0]

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