GGA3 Antibody - #DF13609
| 製品: | GGA3 Antibody |
| カタログ: | DF13609 |
| タンパク質の説明: | Rabbit polyclonal antibody to GGA3 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat, Monkey |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 78kDa; 78kD(Calculated). |
| ユニプロット: | Q9NZ52 |
| RRID: | AB_2846628 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13609, RRID:AB_2846628.
折りたたみ／展開
ADP ribosylation factor binding protein 3; ADP ribosylation factor binding protein GGA 3; ADP ribosylation factor binding protein GGA3; ADP-ribosylation factor-binding protein GGA3; ARF binding protein GGA 3; ARF binding protein GGA3; ARF-binding protein 3; gamma ear-containing; GGA 3; GGA3; GGA3_HUMAN; Golgi associated gamma adaptin ear containing ARF binding protein 3; Golgi localized gamma ear containing ARF binding protein 3; Golgi-localized; KIAA0154;
免疫原
- Q9NZ52 GGA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEAEGESLESWLNKATNPSNRQEDWEYIIGFCDQINKELEGPQIAVRLLAHKIQSPQEWEALQALTVLEACMKNCGRRFHNEVGKFRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTMALPEEAKIKDAYHMLKRQGIVQSDPPIPVDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLKSKNPDDLQEANKLIKSMVKEDEARIQKVTKRLHTLEEVNNNVRLLSEMLLHYSQEDSSDGDRELMKELFDQCENKRRTLFKLASETEDNDNSLGDILQASDNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNSQCSNQGTLIDLAELDTTNSLSSVLAPAPTPPSSGIPILPPPPQASGPPRSRSSSQAEATLGPSSTSNALSWLDEELLCLGLADPAPNVPPKESAGNSQWHLLQREQSDLDFFSPRPGTAACGASDAPLLQPSAPSSSSSQAPLPPPFPAPVVPASVPAPSAGSSLFSTGVAPALAPKVEPAVPGHHGLALGNSALHHLDALDQLLEEAKVTSGLVKPTTSPLIPTTTPARPLLPFSTGPGSPLFQPLSFQSQGSPPKGPELSLASIHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVSMLNTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPLKEKVRLRYKLTFALGEQLSTEVGEVDQFPPVEQWGNL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NZ52 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K15 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| R548 | Methylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| K592 | Ubiquitination | Uniprot |
研究背景
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. nvolved in BACE1 transport and sorting as well as regulation of BACE1 protein levels. Regulates retrograde transport of BACE1 from endosomes to the trans-Golgi network via interaction through the VHS motif and dependent of BACE1 phosphorylation. Modulates BACE1 protein levels intedepently of the interaction between VHS domain and DXXLL motif through recognition of ubiquitination. Key player in a novel DXXLL-mediated endosomal sorting machinery to the recycling pathway that targets NTRK1 to the plasma membrane (By similarity).
Phosphorylated by CK2 and dephosphorylated by PP2A (By similarity). Phosphorylation of GGA3 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals.
Ubiquitinated.
Proteolytically cleaved during apoptosis by CASP3.
Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein. Endosome membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein. Recycling endosome membrane>Peripheral membrane protein.
Ubiquitously expressed.
Monomer (Probable). Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs, such as ARF1, ARF5 and ARF6. Binds RABEP1 and RABGEF1. Interacts with the membrane proteins M6PR/CD-MPR and IGF2R/CI-MPR and the accessory proteins SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Interacts with ADRA2B. Interacts with NTRK1; the interaction is independent of NTRK1 activation and ubiquitination. Interacts (via VHS domain) with BACE1 (via DXXLL motif).
The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif).
The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis.
The unstructured hinge region contains clathrin-binding and an autoinhibitory (DXXLL) motifs.
The GAE domain binds accessory proteins regulating GGAs function.
Belongs to the GGA protein family.
研究領域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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