Amino-terminal enhancer of split Antibody - #DF13613
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13613, RRID:AB_2846632.
折りたたみ／展開
Aes 1; Aes 2; aes; AES_HUMAN; Aes1; Aes2; Amino enhancer of split; Amino terminal enhancer of split; Amino-terminal enhancer of split; Esp 1; Esp1; Gp130 associated protein GAM; Gp130-associated protein GAM; GRG 5; GRG; GRG protein; Grg-5; GRG5; Groucho-related protein 5; Protein ESP 1; Protein ESP1; Protein GRG; TLE 5; TLE5;
免疫原
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
- Q08117 TLE5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMFPQSRHSGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLTPLPVGLQPPSLPAVSAGTGLLSLSALGSQAHLSKEDKNGHDGDTHQEDDGEKSD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q08117 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R7 | Methylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot |
研究背景
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
Ubiquitinated by XIAP/BIRC4.
Nucleus.
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Homooligomer and heterooligomer with other family members. Binds TCF7 (By similarity). Binds the NF-kappa-B subunit RELA. Interacts with PHF12. Interacts (via Q domain) with SIX3. Interacts with SIX6.
Lacks the C-terminal WD repeats.
Belongs to the WD repeat Groucho/TLE family.
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