PARN Antibody - #DF13623
| 製品: | PARN Antibody |
| カタログ: | DF13623 |
| タンパク質の説明: | Rabbit polyclonal antibody to PARN |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 73kDa; 73kD(Calculated). |
| ユニプロット: | O95453 |
| RRID: | AB_2846642 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13623, RRID:AB_2846642.
折りたたみ／展開
DAN; Deadenylating nuclease; Deadenylation nuclease; PARN; PARN_HUMAN; Poly A specific ribonuclease; Poly(A) specific ribonuclease; Poly(A)-specific ribonuclease PARN; Polyadenylate specific ribonuclease; Polyadenylate-specific ribonuclease;
免疫原
- O95453 PARN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFNPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSFLSPPKIHVSARSKLIEPFFNKLFLMRVMDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQVKIAVNTSKYAESYRIQTYAEYMGRKQEEKQIKRKWTEDSWKEADSKRLNPQCIPYTLQNHYYRNNSFTAPSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEPLSEGRKKAKKLKRMKKELSPAGSISKNSPATLFEVPDTW
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95453 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K220 | Acetylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| Y238 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| K243 | Acetylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| K499 | Acetylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| Y552 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| S562 | Phosphorylation | Uniprot | |
| K566 | Acetylation | Uniprot | |
| K566 | Ubiquitination | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| T589 | Phosphorylation | Uniprot | |
| S619 | Phosphorylation | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| T631 | Phosphorylation | Uniprot | |
| T638 | Phosphorylation | Uniprot |
研究背景
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Also able to recognize and trim poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability.
Phosphorylation by MAPKAPK2, preventing GADD45A mRNA degradation after genotoxic stress.
Nucleus. Cytoplasm. Nucleus>Nucleolus.
Note: Some nuclear fraction is nucleolar.
Ubiquitous.
Homodimer. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B. Interacts with KHSRP. Interacts with CELF1/CUGBP1. Interacts with ZC3HAV1 in an RNA-independent manner. Interacts with DHX36.
Belongs to the CAF1 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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