IRF8 Antibody - #DF13627
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13627, RRID:AB_2846646.
折りたたみ／展開
H ICSBP; H-ICSBP; HGNC:5358; HICSBP; ICSBP 1; ICSBP; ICSBP1; Interferon consensus sequence binding protein 1; Interferon consensus sequence binding protein; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF 8; IRF-8; Irf8; IRF8_HUMAN; MYLS;
免疫原
- Q02556 IRF8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MCDRNGGRRLRQWLIEQIDSSMYPGLIWENEEKSMFRIPWKHAGKQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTDRSQLDISEPYKVYRIVPEEEQKCKLGVATAGCVNEVTEMECGRSEIDELIKEPSVDDYMGMIKRSPSPPEACRSQLLPDWWAQQPSTGVPLVTGYTTYDAHHSAFSQMVISFYYGGKLVGQATTTCPEGCRLSLSQPGLPGTKLYGPEGLELVRFPPADAIPSERQRQVTRKLFGHLERGVLLHSSRQGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTSQFFRELQQFYNSQGRLPDGRVVLCFGEEFPDMAPLRSKLILVQIEQLYVRQLAEEAGKSCGAGSVMQAPEEPPPDQVFRMFPDICASHQRSFFRENQQITV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q02556 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y107 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| Y211 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K269 | Methylation | Uniprot | |
| Y373 | Phosphorylation | Uniprot |
研究背景
Plays a role as a transcriptional activator or repressor. Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Positively regulates macroautophagy in dendritic cells.
Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (By similarity). Ubiquitination leads to its degradation by the proteasome.
Sumoylated with SUMO3. Desumoylated by SENP1.
Nucleus. Cytoplasm.
Note: In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction.
Predominantly expressed in lymphoid tissues.
Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with COPS2.
Belongs to the IRF family.
研究領域
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
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