Smg1 Antibody - #DF13628
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13628, RRID:AB_2846647.
折りたたみ／展開
61E3.4; ATX; hSMG-1; hSMG1; KIAA0421; Lambda interacting protein; Lambda-interacting protein; Lambda/iota protein kinase C interacting protein; Lambda/iota protein kinase C-interacting protein; LIP; Phosphatidylinositol 3 kinase related kinase; Phosphatidylinositol 3 kinase related protein kinase; PI 3 kinase related kinase SMG 1; Serine/threonine protein kinase SMG1; Serine/threonine-protein kinase SMG1; SMG-1; smg1; SMG1_HUMAN;
免疫原
Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
- Q96Q15 SMG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRRAPGSRLSSGGGGGGTKYPRSWNDWQPRTDSASADPDNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYSDESRLSNLLRRITREDDRDRRLATVKQLKEFIQQPENKLVLVKQLDNILAAVHDVLNESSKLLQELRQEGACCLGLLCASLSYEAEKIFKWIFSKFSSSAKDEVKLLYLCATYKALETVGEKKAFSSVMQLVMTSLQSILENVDTPELLCKCVKCILLVARCYPHIFSTNFRDTVDILVGWHIDHTQKPSLTQQVSGWLQSLEPFWVADLAFSTTLLGQFLEDMEAYAEDLSHVASGESVDEDVPPPSVSLPKLAALLRVFSTVVRSIGERFSPIRGPPITEAYVTDVLYRVMRCVTAANQVFFSEAVLTAANECVGVLLGSLDPSMTIHCDMVITYGLDQLENCQTCGTDYIISVLNLLTLIVEQINTKLPSSFVEKLFIPSSKLLFLRYHKEKEVVAVAHAVYQAVLSLKNIPVLETAYKLILGEMTCALNNLLHSLQLPEACSEIKHEAFKNHVFNVDNAKFVVIFDLSALTTIGNAKNSLIGMWALSPTVFALLSKNLMIVHSDLAVHFPAIQYAVLYTLYSHCTRHDHFISSSLSSSSPSLFDGAVISTVTTATKKHFSIILNLLGILLKKDNLNQDTRKLLMTWALEAAVLMKKSETYAPLFSLPSFHKFCKGLLANTLVEDVNICLQACSSLHALSSSLPDDLLQRCVDVCRVQLVHSGTRIRQAFGKLLKSIPLDVVLSNNNHTEIQEISLALRSHMSKAPSNTFHPQDFSDVISFILYGNSHRTGKDNWLERLFYSCQRLDKRDQSTIPRNLLKTDAVLWQWAIWEAAQFTVLSKLRTPLGRAQDTFQTIEGIIRSLAAHTLNPDQDVSQWTTADNDEGHGNNQLRLVLLLQYLENLEKLMYNAYEGCANALTSPPKVIRTFFYTNRQTCQDWLTRIRLSIMRVGLLAGQPAVTVRHGFDLLTEMKTTSLSQGNELEVTIMMVVEALCELHCPEAIQGIAVWSSSIVGKNLLWINSVAQQAEGRFEKASVEYQEHLCAMTGVDCCISSFDKSVLTLANAGRNSASPKHSLNGESRKTVLSKPTDSSPEVINYLGNKACECYISIADWAAVQEWQNAIHDLKKSTSSTSLNLKADFNYIKSLSSFESGKFVECTEQLELLPGENINLLAGGSKEKIDMKKLLPNMLSPDPRELQKSIEVQLLRSSVCLATALNPIEQDQKWQSITENVVKYLKQTSRIAIGPLRLSTLTVSQSLPVLSTLQLYCSSALENTVSNRLSTEDCLIPLFSEALRSCKQHDVRPWMQALRYTMYQNQLLEKIKEQTVPIRSHLMELGLTAAKFARKRGNVSLATRLLAQCSEVQLGKTTTAQDLVQHFKKLSTQGQVDEKWGPELDIEKTKLLYTAGQSTHAMEMLSSCAISFCKSVKAEYAVAKSILTLAKWIQAEWKEISGQLKQVYRAQHQQNFTGLSTLSKNILTLIELPSVNTMEEEYPRIESESTVHIGVGEPDFILGQLYHLSSVQAPEVAKSWAALASWAYRWGRKVVDNASQGEGVRLLPREKSEVQNLLPDTITEEEKERIYGILGQAVCRPAGIQDEDITLQITESEDNEEDDMVDVIWRQLISSCPWLSELDESATEGVIKVWRKVVDRIFSLYKLSCSAYFTFLKLNAGQIPLDEDDPRLHLSHRVEQSTDDMIVMATLRLLRLLVKHAGELRQYLEHGLETTPTAPWRGIIPQLFSRLNHPEVYVRQSICNLLCRVAQDSPHLILYPAIVGTISLSSESQASGNKFSTAIPTLLGNIQGEELLVSECEGGSPPASQDSNKDEPKSGLNEDQAMMQDCYSKIVDKLSSANPTMVLQVQMLVAELRRVTVLWDELWLGVLLQQHMYVLRRIQQLEDEVKRVQNNNTLRKEEKIAIMREKHTALMKPIVFALEHVRSITAAPAETPHEKWFQDNYGDAIENALEKLKTPLNPAKPGSSWIPFKEIMLSLQQRAQKRASYILRLEEISPWLAAMTNTEIALPGEVSARDTVTIHSVGGTITILPTKTKPKKLLFLGSDGKSYPYLFKGLEDLHLDERIMQFLSIVNTMFATINRQETPRFHARHYSVTPLGTRSGLIQWVDGATPLFGLYKRWQQREAALQAQKAQDSYQTPQNPGIVPRPSELYYSKIGPALKTVGLSLDVSRRDWPLHVMKAVLEELMEATPPNLLAKELWSSCTTPDEWWRVTQSYARSTAVMSMVGYIIGLGDRHLDNVLIDMTTGEVVHIDYNVCFEKGKSLRVPEKVPFRMTQNIETALGVTGVEGVFRLSCEQVLHIMRRGRETLLTLLEAFVYDPLVDWTAGGEAGFAGAVYGGGGQQAESKQSKREMEREITRSLFSSRVAEIKVNWFKNRDEMLVVLPKLDGSLDEYLSLQEQLTDVEKLQGKLLEEIEFLEGAEGVDHPSHTLQHRYSEHTQLQTQQRAVQEAIQVKLNEFEQWITHYQAAFNNLEATQLASLLQEISTQMDLGPPSYVPATAFLQNAGQAHLISQCEQLEGEVGALLQQRRSVLRGCLEQLHHYATVALQYPKAIFQKHRIEQWKTWMEELICNTTVERCQELYRKYEMQYAPQPPPTVCQFITATEMTLQRYAADINSRLIRQVERLKQEAVTVPVCEDQLKEIERCIKVFLHENGEEGSLSLASVIISALCTLTRRNLMMEGAASSAGEQLVDLTSRDGAWFLEELCSMSGNVTCLVQLLKQCHLVPQDLDIPNPMEASETVHLANGVYTSLQELNSNFRQIIFPEALRCLMKGEYTLESMLHELDGLIEQTTDGVPLQTLVESLQAYLRNAAMGLEEETHAHYIDVARLLHAQYGELIQPRNGSVDETPKMSAGQMLLVAFDGMFAQVETAFSLLVEKLNKMEIPIAWRKIDIIREARSTQVNFFDDDNHRQVLEEIFFLKRLQTIKEFFRLCGTFSKTLSGSSSLEDQNTVNGPVQIVNVKTLFRNSCFSEDQMAKPIKAFTADFVRQLLIGLPNQALGLTLCSFISALGVDIIAQVEAKDFGAESKVSVDDLCKKAVEHNIQIGKFSQLVMNRATVLASSYDTAWKKHDLVRRLETSISSCKTSLQRVQLHIAMFQWQHEDLLINRPQAMSVTPPPRSAILTSMKKKLHTLSQIETSIATVQEKLAALESSIEQRLKWAGGANPALAPVLQDFEATIAERRNLVLKESQRASQVTFLCSNIIHFESLRTRTAEALNLDAALFELIKRCQQMCSFASQFNSSVSELELRLLQRVDTGLEHPIGSSEWLLSAHKQLTQDMSTQRAIQTEKEQQIETVCETIQNLVDNIKTVLTGHNRQLGDVKHLLKAMAKDEEAALADGEDVPYENSVRQFLGEYKSWQDNIQTVLFTLVQAMGQVRSQEHVEMLQEITPTLKELKTQSQSIYNNLVSFASPLVTDATNECSSPTSSATYQPSFAAAVRSNTGQKTQPDVMSQNARKLIQKNLATSADTPPSTVPGTGKSVACSPKKAVRDPKTGKAVQERNSYAVSVWKRVKAKLEGRDVDPNRRMSVAEQVDYVIKEATNLDNLAQLYEGWTAWV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96Q15 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T19 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K43 | Methylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| R50 | Methylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| K131 | Acetylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K173 | Acetylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| Y252 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| Y257 | Phosphorylation | Uniprot | |
| T262 | Phosphorylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| Y549 | Phosphorylation | Uniprot | |
| K598 | Ubiquitination | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| T637 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| S708 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| K819 | Ubiquitination | Uniprot | |
| K879 | Ubiquitination | Uniprot | |
| T900 | Phosphorylation | Uniprot | |
| Y995 | Phosphorylation | Uniprot | |
| Y998 | Phosphorylation | Uniprot | |
| T1006 | Phosphorylation | Uniprot | |
| S1007 | Phosphorylation | Uniprot | |
| T1047 | Phosphorylation | Uniprot | |
| K1174 | Ubiquitination | Uniprot | |
| K1215 | Ubiquitination | Uniprot | |
| S1218 | Phosphorylation | Uniprot | |
| S1221 | Phosphorylation | Uniprot | |
| K1232 | Ubiquitination | Uniprot | |
| K1241 | Ubiquitination | Uniprot | |
| K1265 | Ubiquitination | Uniprot | |
| K1287 | Acetylation | Uniprot | |
| K1287 | Ubiquitination | Uniprot | |
| K1411 | Ubiquitination | Uniprot | |
| K1455 | Ubiquitination | Uniprot | |
| K1467 | Ubiquitination | Uniprot | |
| K1468 | Ubiquitination | Uniprot | |
| K1478 | Ubiquitination | Uniprot | |
| K1513 | Acetylation | Uniprot | |
| K1516 | Acetylation | Uniprot | |
| K1516 | Ubiquitination | Uniprot | |
| Y1519 | Phosphorylation | Uniprot | |
| T1527 | Phosphorylation | Uniprot | |
| K1544 | Ubiquitination | Uniprot | |
| K1632 | Ubiquitination | Uniprot | |
| S1638 | Phosphorylation | Uniprot | |
| K1650 | Ubiquitination | Uniprot | |
| T1660 | Phosphorylation | Uniprot | |
| K1666 | Ubiquitination | Uniprot | |
| T1789 | Phosphorylation | Uniprot | |
| K1798 | Ubiquitination | Uniprot | |
| S1897 | Phosphorylation | Uniprot | |
| S1903 | Phosphorylation | Uniprot | |
| S1907 | Phosphorylation | Uniprot | |
| S1910 | Phosphorylation | Uniprot | |
| S1917 | Phosphorylation | Uniprot | |
| K1989 | Ubiquitination | Uniprot | |
| K2038 | Ubiquitination | Uniprot | |
| K2063 | Ubiquitination | Uniprot | |
| S2087 | Phosphorylation | Uniprot | |
| K2138 | Acetylation | Uniprot | |
| K2139 | Acetylation | Uniprot | |
| K2139 | Ubiquitination | Uniprot | |
| K2148 | Acetylation | Uniprot | |
| S2149 | Phosphorylation | Uniprot | |
| Y2150 | Phosphorylation | Uniprot | |
| Y2152 | Phosphorylation | Uniprot | |
| Y2193 | Phosphorylation | Uniprot | |
| K2232 | Ubiquitination | Uniprot | |
| T2239 | Phosphorylation | Uniprot | |
| Y2253 | Phosphorylation | Uniprot | |
| Y2254 | Phosphorylation | Uniprot | |
| S2255 | Phosphorylation | Uniprot | |
| K2256 | Ubiquitination | Uniprot | |
| S2267 | Phosphorylation | Uniprot | |
| K2370 | Ubiquitination | Uniprot | |
| K2471 | Ubiquitination | Uniprot | |
| K2507 | Ubiquitination | Uniprot | |
| K2511 | Ubiquitination | Uniprot | |
| Y2536 | Phosphorylation | Uniprot | |
| S2537 | Phosphorylation | Uniprot | |
| K2658 | Ubiquitination | Uniprot | |
| Y2713 | Phosphorylation | Uniprot | |
| K2729 | Ubiquitination | Uniprot | |
| T2776 | Phosphorylation | Uniprot | |
| S2955 | Phosphorylation | Uniprot | |
| K3030 | Ubiquitination | Uniprot | |
| K3140 | Ubiquitination | Uniprot | |
| T3181 | Phosphorylation | Uniprot | |
| T3245 | Phosphorylation | Uniprot | |
| K3426 | Ubiquitination | Uniprot | |
| S3544 | Phosphorylation | Uniprot | |
| T3546 | Phosphorylation | Uniprot | |
| T3550 | Phosphorylation | Uniprot | |
| S3556 | Phosphorylation | Uniprot | |
| T3569 | Phosphorylation | Uniprot | |
| S3570 | Phosphorylation | Uniprot | |
| T3573 | Phosphorylation | Uniprot | |
| S3576 | Phosphorylation | Uniprot | |
| T3577 | Phosphorylation | Uniprot | |
| S3588 | Phosphorylation | Uniprot | |
| K3619 | Methylation | Uniprot | |
| S3632 | Phosphorylation | Uniprot |
PTMs - Q96Q15 酵素として
研究背景
Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
Autophosphorylated.
Nucleus. Cytoplasm.
Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2. Interacts with UPF2.
Belongs to the PI3/PI4-kinase family.
研究領域
· Genetic Information Processing > Translation > mRNA surveillance pathway.
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