E1 Ubiquitin Activating Enzyme Antibody - #DF13630
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13630, RRID:AB_2846649.
折りたたみ／展開
A1S9; A1S9 protein; A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; CFAP124; CTD-2522E6.1; GXP 1; GXP1; MGC4781; POC20; POC20 centriolar protein homolog; Protein A1S9; SMAX2; Uba1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1_HUMAN; UBA1A; UBE 1; UBE 1X; UBE1; UBE1X; Ubiquitin activating enzyme E1; Ubiquitin-activating enzyme E1; Ubiquitin-like modifier-activating enzyme 1;
免疫原
- P22314 UBA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P22314 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S7 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| K68 | Acetylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| R81 | Methylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| R119 | Methylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| C234 | S-Nitrosylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| R288 | Methylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K304 | Methylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| R368 | Methylation | Uniprot | |
| R384 | Methylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| K465 | Acetylation | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K470 | Acetylation | Uniprot | |
| K470 | Ubiquitination | Uniprot | |
| C481 | S-Nitrosylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K528 | Sumoylation | Uniprot | |
| K528 | Ubiquitination | Uniprot | |
| R551 | Methylation | Uniprot | |
| R558 | Methylation | Uniprot | |
| Y560 | Phosphorylation | Uniprot | |
| C588 | S-Nitrosylation | Uniprot | |
| Y590 | Phosphorylation | Uniprot | |
| K593 | Acetylation | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| T600 | Phosphorylation | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| K604 | Acetylation | Uniprot | |
| K604 | Sumoylation | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| T615 | Phosphorylation | Uniprot | |
| K627 | Acetylation | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| C632 | S-Nitrosylation | Uniprot | |
| K635 | Sumoylation | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| K671 | Acetylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| R678 | Methylation | Uniprot | |
| T682 | Phosphorylation | Uniprot | |
| K746 | Ubiquitination | Uniprot | |
| S781 | Phosphorylation | Uniprot | |
| T789 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| T800 | Phosphorylation | Uniprot | |
| K802 | Ubiquitination | Uniprot | |
| S803 | Phosphorylation | Uniprot | |
| K806 | Ubiquitination | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| S816 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| S824 | Phosphorylation | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| T832 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K838 | Acetylation | Uniprot | |
| K838 | Ubiquitination | Uniprot | |
| K843 | Ubiquitination | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| Y873 | Phosphorylation | Uniprot | |
| R880 | Methylation | Uniprot | |
| K889 | Ubiquitination | Uniprot | |
| K923 | Ubiquitination | Uniprot | |
| R957 | Methylation | Uniprot | |
| Y978 | Phosphorylation | Uniprot | |
| K980 | Acetylation | Uniprot | |
| K980 | Ubiquitination | Uniprot | |
| K1024 | Acetylation | Uniprot | |
| C1039 | S-Nitrosylation | Uniprot | |
| C1040 | S-Nitrosylation | Uniprot | |
| S1044 | Phosphorylation | Uniprot |
研究背景
Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
ISGylated.
Cytoplasm. Mitochondrion. Nucleus.
Nucleus.
Cytoplasm.
Detected in erythrocytes (at protein level). Ubiquitous.
Monomer (By similarity). Interacts with GAN (via BTB domain).
The first 11 amino acids are essential for phosphorylation and exclusive nuclear localization.
Belongs to the ubiquitin-activating E1 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
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