Hhip Antibody - #DF13634

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製品: Hhip Antibody
カタログ: DF13634
タンパク質の説明: Rabbit polyclonal antibody to Hhip
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 78kDa; 79kD(Calculated).
ユニプロット: Q96QV1
RRID: AB_2846653

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
Hhip Antibody detects endogenous levels of total Hhip.
RRID:
AB_2846653
引用形式: Affinity Biosciences Cat# DF13634, RRID:AB_2846653.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ20992; FLJ90230; Hedgehog interacting protein; Hedgehog-interacting protein; hedgehoginteracting protein; HHIP; HHIP_HUMAN; Hip;

免疫原

免疫原:

A synthesized peptide derived from human Hhip, corresponding to a region within C-terminal amino acids.

Uniprot:

Q96QV1(HHIP_HUMAN) >>Visit HPA database.

遺伝子(ID):
HHIP(64399)
発現特異性:
Q96QV1 HHIP_HUMAN:

Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney.

タンパク質配列:
MLKMLSFKLLLLAVALGFFEGDAKFGERNEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGGFYPRLSCCLRSDSPGLGRLENKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREVLERDLVLPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQVRGPASNYLDQMEEYDKVEEISRKHKHNCFCIQEVVSGLRQPVGALHSGDGSQRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCNVPYSIPRSNPHFNSTNQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVTKQWQEKPLCLGTSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRATVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRTAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRAGILDQIIDMTSYLLDLTSYIV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.

細胞の位置付け:

Cell membrane>Peripheral membrane protein. Secreted.
Note: The last 22 C-terminal amino acids may participate in cell membrane attachment.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed in fetal and adult tissues. Highest expression in adult heart, liver and pancreas, and in fetal kidney.

タンパク質ファミリー:

A flexible loop interacts with the SHH zinc binding site and contributes to zinc binding.

Belongs to the HHIP family.

研究領域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

参考文献

1). LncRNA HHIP-AS1 suppresses lung squamous cell carcinoma by stabilizing HHIP mRNA. Life sciences, 2023 (PubMed: 36958438) [IF=5.2]
2). Identification by RNA-Seq of let-7 clusters as prenatal biomarkers for nonsyndromic cleft lip with palate. Annals of the New York Academy of Sciences, 2022 (PubMed: 35854669) [IF=4.1]
3). CircFAM114A2 inhibits the progression of hepatocellular carcinoma via miR-630/HHIP axis. Cancer medicine, 2023 (PubMed: 37039160) [IF=2.9]

Application: IHC    Species: human    Sample:

FIGURE 6 CircFAM114A2 suppresses HCC progression through miR-630/HHIP axis. (A) Relative expression of miR-630 were detected by qRT-PCR in circFAM114A2 silenced or overexpressed cells. (B) Relative expression of HHIP mRNA in miR-630 overexpressed cells. (C) The silenced efficiency of HHIP was detected by qRT-PCR. (D) EdU assays were used to evaluate cell proliferation after co-transfection with circFAM114A2 overexpression plasmid and HHIP siRNA. Scale bars, 200 μm. (E) CCK-8 assays were used to evaluate cell viability after co-transfection. (F) EdU assays were used to evaluate cell proliferation after co-transfection with circFAM114A2 overexpression plasmid and miR-630 mimic. Scale bars, 200 μm. (G) CCK-8 assays were used to evaluate cell viability after co-transfection. (H) IHC analysis of the expression of HHIP. The figures were imaged at 100× and 400× magnification. Scale bar, 100 μm and 20 μm. **p 

Application: WB    Species: human    Sample:

FIGURE 5 CircFAM114A2 upregulates HHIP expression via “miR-630 sponge”. (A) RAP experiment's schematic diagram (B–D) Following the RAP experiment, qRT-PCR was used to determine the enrichment of circFAM114A2 and potential miRNAs. The qRT-PCR products were then processed to nucleic acid gel electrophoresis using a 1% agarose gel. (E) Schematic diagram of binding sites between miR-630 and circFAM114A2 or its mutant type (circFAM114A2 MUT). (F) Luciferase reporter assay was performed to validate the interaction between circFAM114A2 and miR-630. (G) The TargetScan, miRmap and RNA Interactome databases were used to predict miR-630 targeted mRNAs. (H) Relative expression levels of HHIP was detected in circFAM114A2 silenced and overexpressed cells. (I) Protein level of HHIP was detected by western blot. (J) Schematic diagram of binding sites between miR-630 and HHIP or its mutant type (HHIP-MUT). (K) Luciferase reporter assay was performed in 293 T cells to validate the interaction between HHIP and miR-630. (L) Survival analysis of the expression of HHIP between clinic PFC and DFS. (M) Relative expression of HHIP in HCC tissues. (N) Relative expression of miR-630 in HCC tissues. **p 
4). 7-Dehydrocholesterol Reductase Activates the Hedgehog Pathway by Regulating Cholesterol to Promote the Development of Triple-Negative Breast Cancer. Current cancer drug targets, 2025 (PubMed: 39957696) [IF=2.3]

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