COL4A3BP Antibody - #DF13647
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13647, RRID:AB_2846666.
折りたたみ／展開
Alpha 3 type IV collagen binding protein; C43BP_HUMAN; Ceramide transfer protein; Ceramide transporter; CERT; Cert protein; CERTL; col4a3bp; Collagen type IV alpha 3 (Goodpasture antigen) binding protein; Collagen type IV alpha 3 binding protein; Collagen type IV alpha-3-binding protein; FLJ20597; Goodpasture antigen binding protein; Goodpasture antigen-binding protein; GPBP; hCERT; Lipid transfer protein CERTL; Procollagen type IV alpha 3 (Goodpasture antigen) binding protein; StAR related lipid transfer (START) domain containing 11; StAR related lipid transfer protein 11; StAR-related lipid transfer protein 11; StARD 11; StARD11; START domain containing 11; START domain containing protein 11; START domain-containing protein 11;
免疫原
- Q9Y5P4 CERT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNALSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPDHRQQWIDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDACADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHSTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREDSWQKRLDKETEKKRRTEEAYKNAMTELKKKSHFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPTSLPSGDAFSSVGTHRFVQKPYSRSSSMSSIDLVSASDDVHRFSSQVEEMVQNHMTYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSVIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINVAMICQTLVSPPEGNQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y5P4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| T117 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S132 | Phosphorylation | P78368 (CSNK1G2) , Q15139 (PRKD1) | Uniprot |
| S135 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| T146 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| T148 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| T232 | Phosphorylation | Uniprot | |
| K242 | Acetylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| Y372 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| Y579 | Phosphorylation | Uniprot | |
| T610 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot |
研究背景
Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.
Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. Inactivated by hyperphosphorylation of serine residues by CSNK1G2/CK1 that triggers dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis.
Cytoplasm. Golgi apparatus. Endoplasmic reticulum.
Note: Preferentially localized to the Golgi apparatus.
Widely expressed.
Interacts with VAPA and VAPB. Interaction with VAPB is less efficient than with VAPA.
The START domain recognizes ceramides and diacylglycerol lipids, interacts with membranes, and mediates the intermembrane transfer of ceramides and diacylglycerol lipids.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB.
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