PPP2R5C Antibody - #DF13668
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13668, RRID:AB_2846687.
折りたたみ／展開
2A5G_HUMAN; B' alpha regulatory subunit; B56G; KIAA0044; MGC23064; PP2A B subunit B' gamma isoform; PP2A B subunit B56 gamma isoform; PP2A B subunit isoform B' gamma; PP2A B subunit isoform B''-gamma; PP2A B subunit isoform B56-gamma; PP2A B subunit isoform PR61-gamma; PP2A B subunit isoform R5-gamma; PP2A B subunit PR61 gamma isoform; PP2A B subunit R5 gamma isoform; Ppp2r5c; PR61G; Protein phosphatase 2 regulatory subunit B (B56) gamma isoform; Protein phosphatase 2 regulatory subunit B' gamma; Protein phosphatase 2, regulatory subunit B', gamma isoform; Renal carcinoma antigen NY REN 29; Renal carcinoma antigen NY-REN-29; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit gamma isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform;
免疫原
Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
- Q13362 2A5G_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLTCNKAGSRMVVDAANSNGPFQPVVLLHIRDVPPADQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDSTLTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEFVKIMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKLKEKLKMKEREEAWVKIENLAKANPQYTVYSQASTMSIPVAMETDGPLFEDVQMLRKTVKDEAHQAQKDPKKDRPLARRKSELPQDPHTKKALEAHCRADELASQDGR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13362 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| Y79 | Phosphorylation | Uniprot | |
| T88 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K333 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S337 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
| Y348 | Phosphorylation | Uniprot | |
| Y349 | Phosphorylation | Uniprot | |
| Y354 | Phosphorylation | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| T385 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| K487 | Acetylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Q13315 (ATM) | Uniprot |
研究背景
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.
Isoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C.
Nucleus. Chromosome>Centromere.
Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.
PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with PPP2CA AND PPP2R1A; the interaction is direct. Interacts with SGO1; the interaction is direct. Isoform 1 and isoform 2 interact with TP53 (phosphorylated at Ser-15 by ATM); increased upon DNA damage it drives PP2A-mediated dephosphorylation of TP53 at Thr-55. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation. Interacts with CIP2A; this interaction stabilizes CIP2A.
Belongs to the phosphatase 2A regulatory subunit B56 family.
研究領域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Genetic Information Processing > Translation > mRNA surveillance pathway.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
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