VPS36 Antibody - #DF13682
| 製品: | VPS36 Antibody |
| カタログ: | DF13682 |
| タンパク質の説明: | Rabbit polyclonal antibody to VPS36 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 43kDa; 44kD(Calculated). |
| ユニプロット: | Q86VN1 |
| RRID: | AB_2846701 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13682, RRID:AB_2846701.
折りたたみ／展開
C13orf9; CGI 145; EAP45; ELL associated protein of 45 kDa; ELL-associated protein of 45 kDa; ESCRT-II complex subunit VPS36; Vacuolar protein sorting associated protein 36; Vacuolar protein-sorting-associated protein 36; Vps36; VPS36_HUMAN;
免疫原
- Q86VN1 VPS36_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q86VN1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y27 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K183 | Acetylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot |
研究背景
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
Cytoplasm. Endosome. Late endosome. Membrane. Nucleus.
Note: Colocalizes with ubiquitinated proteins on late endosomes. Recruited to the endosome membrane to participate in vesicle formation.
Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25. Interacts with VPS25, SNF8, TSG101 and CHMP6. Interacts (via GLUE domain) with ubiquitin. Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with ECPAS.
The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.
Belongs to the VPS36 family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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