UNC5B Antibody - #DF13685
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| 製品: | UNC5B Antibody |
| カタログ: | DF13685 |
| タンパク質の説明: | Rabbit polyclonal antibody to UNC5B |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 103kDa; 104kD(Calculated). |
| ユニプロット: | Q8IZJ1 |
| RRID: | AB_2846704 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13685, RRID:AB_2846704.
折りたたみ／展開
6330415E02Rik; A630020F16; D10Bwg0792e; Netrin receptor UNC5B; Netrin receptor UNC5B Precursor; p53 regulated receptor for death and life; p53 regulated receptor for death and life protein 1; p53-regulated receptor for death and life protein 1; p53RDL1; Protein unc-5 homolog 2; Protein unc-5 homolog B; Protein XUNC 5; Transmembrane receptor Unc5H2; Unc 5 homolog 2; Unc 5 homolog B; Unc 5 netrin receptor B; UNC 5B; Unc5 (C.elegans homolog) b; Unc5b; UNC5B_HUMAN; UNC5H2;
免疫原
Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues.
- Q8IZJ1 UNC5B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGARSGARGALLLALLLCWDPRLSQAGTDSGSEVLPDSFPSAPAEPLPYFLQEPQDAYIVKNKPVELRCRAFPATQIYFKCNGEWVSQNDHVTQEGLDEATGLRVREVQIEVSRQQVEELFGLEDYWCQCVAWSSAGTTKSRRAYVRIAYLRKNFDQEPLGKEVPLDHEVLLQCRPPEGVPVAEVEWLKNEDVIDPTQDTNFLLTIDHNLIIRQARLSDTANYTCVAKNIVAKRRSTTATVIVYVNGGWSSWAEWSPCSNRCGRGWQKRTRTCTNPAPLNGGAFCEGQAFQKTACTTICPVDGAWTEWSKWSACSTECAHWRSRECMAPPPQNGGRDCSGTLLDSKNCTDGLCMQNKKTLSDPNSHLLEASGDAALYAGLVVAIFVVVAILMAVGVVVYRRNCRDFDTDITDSSAALTGGFHPVNFKTARPSNPQLLHPSVPPDLTASAGIYRGPVYALQDSTDKIPMTNSPLLDPLPSLKVKVYSSSTTGSGPGLADGADLLGVLPPGTYPSDFARDTHFLHLRSASLGSQQLLGLPRDPGSSVSGTFGCLGGRLSIPGTGVSLLVPNGAIPQGKFYEMYLLINKAESTLPLSEGTQTVLSPSVTCGPTGLLLCRPVILTMPHCAEVSARDWIFQLKTQAHQGHWEEVVTLDEETLNTPCYCQLEPRACHILLDQLGTYVFTGESYSRSAVKRLQLAVFAPALCTSLEYSLRVYCLEDTPVALKEVLELERTLGGYLVEEPKPLMFKDSYHNLRLSLHDLPHAHWRSKLLAKYQEIPFYHIWSGSQKALHCTFTLERHSLASTELTCKICVRQVEGEGQIFQLHTTLAETPAGSLDTLCSAPGSTVTTQLGPYAFKIPLSIRQKICNSLDAPNSRGNDWRMLAQKLSMDRYLNYFATKASPTGVILDLWEALQQDDGDLNSLASALEEMGKSEMLVAVATDGDC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IZJ1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T341 | Phosphorylation | Uniprot | |
| Y452 | Phosphorylation | Uniprot | |
| Y457 | Phosphorylation | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| Y511 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| T733 | Phosphorylation | Uniprot | |
| Y737 | Phosphorylation | Uniprot | |
| S800 | Phosphorylation | Uniprot | |
| S803 | Phosphorylation | Uniprot | |
| T804 | Phosphorylation | Uniprot | |
| T807 | Phosphorylation | Uniprot | |
| S869 | Phosphorylation | Uniprot | |
| Y892 | Phosphorylation | Uniprot | |
| Y895 | Phosphorylation | Uniprot |
研究背景
Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity).
Phosphorylated on cytoplasmic tyrosine residues.
Proteolytically cleaved by caspases during apoptosis. The cleavage does not take place when the receptor is associated with netrin ligand. Its cleavage by caspases is required to induce apoptosis.
Palmitoylation is required for pro-apoptotic activity, but not for location at lipid rafts.
Cell membrane>Single-pass type I membrane protein. Membrane raft.
Note: Associated with lipid rafts.
Highly expressed in brain. Also expressed at lower level in developing lung, cartilage, kidney and hematopoietic and immune tissues.
Interacts with the cytoplasmic part of DCC (By similarity). Interacts with GNAI2 via its cytoplasmic part. Interacts (via death domain) with DAPK1 (via death domain). Interacts (via extracellular domain) with FLRT3 (via extracellular domain); the interaction is direct. Interacts (via extracellular domain) with FLRT2 and FLRT3 (via extracellular domain), but has higher affinity for FLRT3. Identified in a complex with FLRT3 and ADGRL3; does not interact with ADGRL3 by itself.
Belongs to the unc-5 family.
研究領域
· Organismal Systems > Development > Axon guidance. (View pathway)
参考文献
Application: WB Species: Human Sample: U251 cells
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