Snx6 Antibody - #DF13700
製品: | Snx6 Antibody |
カタログ: | DF13700 |
タンパク質の説明: | Rabbit polyclonal antibody to Snx6 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 46kDa, 40 kDa; 47kD(Calculated). |
ユニプロット: | Q9UNH7 |
RRID: | AB_2846719 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13700, RRID:AB_2846719.
免疫原
- Q9UNH7 SNX6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMEGLDDGPDFLSEEDRGLKAINVDLQSDAALQVDISDALSERDKVKFTVHTKSSLPNFKQNEFSVVRQHEEFIWLHDSFVENEDYAGYIIPPAPPRPDFDASREKLQKLGEGEGSMTKEEFTKMKQELEAEYLAIFKKTVAMHEVFLCRVAAHPILRRDLNFHVFLEYNQDLSVRGKNKKEKLEDFFKNMVKSADGVIVSGVKDVDDFFEHERTFLLEYHNRVKDASAKSDRMTRSHKSAADDYNRIGSSLYALGTQDSTDICKFFLKVSELFDKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKALDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELKHAKGNLQLLQNCLAVLNGDT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UNH7 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
M2 | Acetylation | Uniprot | |
S13 | Phosphorylation | Uniprot | |
R17 | Methylation | Uniprot | |
K47 | Ubiquitination | Uniprot | |
K53 | Ubiquitination | Uniprot | |
S55 | Phosphorylation | Uniprot | |
K60 | Ubiquitination | Uniprot | |
K109 | Ubiquitination | Uniprot | |
S116 | Phosphorylation | Uniprot | |
K119 | Acetylation | Uniprot | |
K119 | Ubiquitination | Uniprot | |
K124 | Acetylation | Uniprot | |
K126 | Ubiquitination | Uniprot | |
Y133 | Phosphorylation | Uniprot | |
K138 | Ubiquitination | Uniprot | |
T140 | Phosphorylation | Uniprot | |
C149 | S-Nitrosylation | Uniprot | |
K183 | Ubiquitination | Uniprot | |
K189 | Ubiquitination | Uniprot | |
K193 | Ubiquitination | Uniprot | |
S194 | Phosphorylation | Uniprot | |
K204 | Ubiquitination | Uniprot | |
S231 | Phosphorylation | Uniprot | |
K239 | Ubiquitination | Uniprot | |
S250 | Phosphorylation | Uniprot | |
Y253 | Phosphorylation | Uniprot | |
K265 | Ubiquitination | Uniprot | |
K269 | Ubiquitination | Uniprot | |
K276 | Ubiquitination | Uniprot | |
K291 | Ubiquitination | Uniprot | |
K297 | Ubiquitination | Uniprot | |
K307 | Ubiquitination | Uniprot | |
S316 | Phosphorylation | Uniprot | |
Y320 | Phosphorylation | Uniprot | |
K325 | Ubiquitination | Uniprot | |
K335 | Ubiquitination | Uniprot | |
K350 | Ubiquitination | Uniprot | |
K353 | Ubiquitination | Uniprot | |
K359 | Ubiquitination | Uniprot | |
K366 | Ubiquitination | Uniprot | |
K375 | Ubiquitination | Uniprot | |
K386 | Acetylation | Uniprot | |
K386 | Ubiquitination | Uniprot | |
K389 | Ubiquitination | Uniprot |
研究背景
Involved in several stages of intracellular trafficking. Interacts with membranes phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate (Probable). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Does not have in vitro vesicle-to-membrane remodeling activity. Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptor IGF2R. May function as link between transport vesicles and dynactin (Probable). Negatively regulates retrograde transport of BACE1 from the cell surface to the trans-Golgi network. Involved in E-cadherin sorting and degradation; inhibits PIP5K1C isoform 3-mediated E-cadherin degradation. In association with GIT1 involved in EGFR degradation. Promotes lysosomal degradation of CDKN1B (By similarity). May contribute to transcription regulation (Probable).
In vitro phosphorylated by PIM1; not affecting PIM1-dependent nuclear translocation.
Early endosome. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle. Cytoplasm. Nucleus.
Note: Interaction with SNX1 or SNX2 promotes location at endosome membranes (PubMed:19935774). Only a minor proportion is seen in the nucleus.
Forms heterodimers with BAR domain-containing sorting nexins SNX1 and SNX2. The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also descibed as vacuolar protein sorting subcomplex (VPS), and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity (Probable). Interacts with SNX1, SNX2, VPS26A, VPS29, VPS35, CDKN1B, TGFB receptors, BACE1, BRMS1, PIP5K1C isoform 3. Interacts with DCTN1; the association with DCTN1 is involved in movement of retromer-c ontaining vesicles toward the TGN. Interacts with CDKN1B and GIT1 (By similarity). Interacts with PIM1; translocating SNX6 to the nucleus.
The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.
The BAR domain is able to sense membrane curvature upon dimerization. Membrane remodeling seems to implicate insertion of an amphipatric helix (AH) in the membrane (Probable).
Belongs to the sorting nexin family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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