Proteasome Activator Subunit 4 Antibody - #DF13732
| 製品: | Proteasome Activator Subunit 4 Antibody |
| カタログ: | DF13732 |
| タンパク質の説明: | Rabbit polyclonal antibody to Proteasome Activator Subunit 4 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 211kDa; 211kD(Calculated). |
| ユニプロット: | Q14997 |
| RRID: | AB_2846751 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13732, RRID:AB_2846751.
折りたたみ／展開
KIAA0077; PA200; Proteasome Activator 200 kDa; Proteasome activator complex subunit 4; Proteasome activator PA200; PSME 4; psme4; PSME4_HUMAN;
免疫原
- Q14997 PSME4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPAERAGVGEPPEPGGRPEPGPRGFVPQKEIVYNKLLPYAERLDAESDLQLAQIKCNLGRAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLLINLLKKKELLSRADLELPWRPLYDMVERILYSKTEHLGLNWFPNSVENILKTLVKSCRPYFPADATAEMLEEWRPLMCPFDVTMQKAITYFEIFLPTSLPPELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERYKKPSWLTPVPDSHKLTDQDVTDFVQCIIQPVLLAMFSKTGSLEAAQALQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLSCVIGVARSLVSGGRWFPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIATFSTLVPLVDCSSVLQERNDLTEVERELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKEIFMVALQKVFNFSTSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCCSVITQLTMNDDVLNDEELDKELLWNLQLLSEITRVDGRKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHHLLRSTTLIYPTEYCSVPGGFDKPPSEYFPIKDWGKPGDLWNLGIQWHVPSSEEVSFAFYLLDSFLQPELVKLQHCGDGKLEMSRDDILQSLTIVHNCLIGSGNLLPPLKGEPVTNLVPSMVSLEETKLYTGLEYDLSRENHREVIATVIRKLLNHILDNSEDDTKSLFLIIKIIGDLLQFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRQGVTQQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPAIVSSGLSQAMSLEKPSIVRLFDDLAEKIHRQYETIGLDFTIPKSCVEIAELLQQSKNPSINQILLSPEKIKEGIKRQQEKNADALRNYENLVDTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLTINPCEISGCPKPTQIIAGDRPDNHWLHYDSKTIPRTKKEWESSCFVEKTHWGYYTWPKNMVVYAGVEEQPKLGRSREDMTEAEQIIFDHFSDPKFVEQLITFLSLEDRKGKDKFNPRRFCLFKGIFRNFDDAFLPVLKPHLEHLVADSHESTQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNITVETYNDWGACIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTTPTISPHVPEFTARILEKLKPLMDVDEEIQNHVMEENGIGEEDERTQGIKLLKTILKWLMASAGRSFSTAVTEQLQLLPLFFKIAPVENDNSYDELKRDAKLCLSLMSQGLLYPHQVPLVLQVLKQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNEDAVKDIRWLVISLLEDEQLEVREMAATTLSGLLQCNFLTMDSPMQIHFEQLCKTKLPKKRKRDPGSVGDTIPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQEHKQQFTDDQLLVLTDLLVSPCYYA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14997 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K36 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| T81 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T572 | Phosphorylation | Uniprot | |
| S580 | Phosphorylation | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S883 | Phosphorylation | Uniprot | |
| K908 | Ubiquitination | Uniprot | |
| K916 | Ubiquitination | Uniprot | |
| K922 | Ubiquitination | Uniprot | |
| T952 | Phosphorylation | Uniprot | |
| T954 | Phosphorylation | Uniprot | |
| K1082 | Ubiquitination | Uniprot | |
| Y1087 | Phosphorylation | Uniprot | |
| S1121 | Phosphorylation | Uniprot | |
| K1124 | Ubiquitination | Uniprot | |
| S1206 | Phosphorylation | Uniprot | |
| K1261 | Ubiquitination | Uniprot | |
| K1294 | Ubiquitination | Uniprot | |
| S1298 | Phosphorylation | Uniprot | |
| T1394 | Phosphorylation | Uniprot | |
| K1397 | Acetylation | Uniprot | |
| T1411 | Phosphorylation | Uniprot | |
| Y1489 | Phosphorylation | Uniprot | |
| K1493 | Ubiquitination | Uniprot | |
| K1572 | Ubiquitination | Uniprot | |
| K1605 | Ubiquitination | Uniprot | |
| S1614 | Phosphorylation | Uniprot | |
| Y1615 | Phosphorylation | Uniprot | |
| K1619 | Ubiquitination | Uniprot | |
| S1746 | Phosphorylation | Uniprot | |
| S1753 | Phosphorylation | Uniprot | |
| K1758 | Ubiquitination | Uniprot | |
| K1804 | Ubiquitination | Uniprot |
研究背景
Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
Cytoplasm>Cytosol. Nucleus. Nucleus speckle.
Note: Found in nuclear foci following treatment with ionizing radiation, but not with ultraviolet irradiation or H(2)O(2).
Homodimer. Interacts with the 20S and 26S proteasomes. Component of the spermatoproteasome, a form of the proteasome specifically found in testis.
The bromodomain-like (BRDL) region specifically recognizes and binds acetylated histones.
Belongs to the BLM10 family.
研究領域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
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