FLIP Antibody - #DF13733
| 製品: | FLIP Antibody |
| カタログ: | DF13733 |
| タンパク質の説明: | Rabbit polyclonal antibody to FLIP |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Horse, Dog |
| 分子量: | 55kDa; 55kD(Calculated). |
| ユニプロット: | O15519 |
| RRID: | AB_2846752 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13733, RRID:AB_2846752.
折りたたみ／展開
c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8AP1; Caspase homolog; Caspase like apoptosis regulatory protein; Caspase related inducer of apoptosis; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; Casper; Cellular FLICE-like inhibitory protein; CFLA; Cflar; CFLAR_HUMAN; CLARP; FADD like anti apoptotic molecule; FADD-like antiapoptotic molecule 1; FLAME; FLAME-1; FLAME1; FLIP; I FLICE; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; OTTHUMP00000163715; OTTHUMP00000206475; OTTHUMP00000206476; OTTHUMP00000206478; OTTHUMP00000206479; OTTHUMP00000206480; OTTHUMP00000206482; OTTHUMP00000207360; Usurpin; Usurpin beta;
免疫原
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
- O15519 CFLAR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVLMAEIGEDLDKSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQSVQGAGTSYRNVLQAAIQKSLKDPSNNFRLHNGRSKEQRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYKMKSKPLGICLIIDCIGNETELLRDTFTSLGYEVQKFLHLSMHGISQILGQFACMPEHRDYDSFVCVLVSRGGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAGKPKMFFIQNYVVSEGQLEDSSLLEVDGPAMKNVEFKAQKRGLCTVHREADFFWSLCTADMSLLEQSHSSPSLYLQCLSQKLRQERKRPLLDLHIELNGYMYDWNSRVSAKEKYYVWLQHTLRKKLILSYT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O15519 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K49 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| R122 | Methylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| S193 | Phosphorylation | P17252 (PRKCA) , P05771 (PRKCB) | Uniprot |
| K195 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| C254 | S-Nitrosylation | Uniprot | |
| C259 | S-Nitrosylation | Uniprot | |
| S273 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S314 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot |
研究背景
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Proteolytically processed by CASP8 generating subunit p43 and p12.
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
TNFRSF6 stimulation triggers recruitment to the death-inducing signaling complex (DISC) formed by TNFRSF6, FADD and CASP8. A proteolytic fragment (p43) stays associated with the DISC. Also interacts with FADD, CASP8, CASP3, TRAF1, TRAF2 and Bcl-X(L) (in vitro). Interacts with RIPK1 (By similarity).
(Microbial infection) Interacts with HBV protein X.
The caspase domain lacks the active site residues involved in catalysis.
Belongs to the peptidase C14A family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).
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