PAG Antibody - #DF13738
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13738, RRID:AB_2846757.
折りたたみ／展開
CBP; Csk binding protein; Csk-binding protein; FLJ37858; MGC138364; PAG 1; PAG; PAG1; PAG1_HUMAN; Phosphoprotein associated with glycosphingolipid enriched microdomains 1; Phosphoprotein associated with glycosphingolipid enriched microdomains; Phosphoprotein associated with glycosphingolipid microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Protein Associated with Glycosphingolipid Enriched Microdomains; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp;
免疫原
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
- Q9NWQ8 PHAG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPAGSLLGSGQMQITLWGSLAAVAIFFVITFLIFLCSSCDREKKPRQHSGDHENLMNVPSDKEMFSRSVTSLATDAPASSEQNGALTNGDILSEDSTLTCMQHYEEVQTSASDLLDSQDSTGKPKCHQSRELPRIPPESAVDTMLTARSVDGDQGLGMEGPYEVLKDSSSQENMVEDCLYETVKEIKEVAAAAHLEKGHSGKAKSTSASKELPGPQTEGKAEFAEYASVDRNKKCRQSVNVESILGNSCDPEEEAPPPVPVKLLDENENLQEKEGGEAEESATDTTSETNKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQLVNKSGQSLTVPESTYTSIQGDPQRSPSSCNDLYATVKDFEKTPNSTLPPAGRPSEEPEPDYEAIQTLNREEEKATLGTNGHHGLVPKENDYESISDLQQGRDITRL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NWQ8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S50 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| T98 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | P07948 (LYN) | Uniprot |
| S150 | Phosphorylation | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| Y299 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| T307 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y317 | Phosphorylation | P12931 (SRC) | Uniprot |
| S318 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| T342 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S354 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| T361 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| T392 | Phosphorylation | Uniprot | |
| T404 | Phosphorylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot |
研究背景
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
Palmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN.
Cell membrane>Single-pass type III membrane protein.
Note: Present in lipid rafts.
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
Interacts with FYN. When phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Interacts with LYN on plasma membrane lipid rafts. Identified in a complex with LYN and STAT3.
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