製品: ITK Antibody
カタログ: AF0003
タンパク質の説明: Rabbit polyclonal antibody to ITK
アプリケーション: ELISA(peptide)
反応性: Human, Mouse, Rat
分子量: 68kDa; 72kD(Calculated).
ユニプロット: Q08881
RRID: AB_2846781

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製品説明

ソース:
Rabbit
アプリケーション:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
ITK Antibody detects endogenous levels of total ITK.
RRID:
AB_2846781
引用形式: Affinity Biosciences Cat# AF0003, RRID:AB_2846781.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

EMT; Homolog of mouse T cell itk/tsk; IL 2 inducible T cell kinase; IL2 inducible T cell kinase; Interleukin 2 inducible T cell kinase; Itk; ITK_HUMAN; Kinase EMT; LPFS1; LYK; MGC126257; MGC126258; PSCTK 2; PSCTK2; T cell specific kinase; T-cell-specific kinase; TSK; Tyrosine protein kinase; Tyrosine protein kinase ITK/TSK; Tyrosine protein kinase Lyk; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk;

免疫原

免疫原:

A synthesized peptide derived from human ITK

Uniprot:
遺伝子(ID):
発現特異性:
Q08881 ITK_HUMAN:

T-cell lines and natural killer cell lines.

タンパク質配列:
MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPKYHPNFWMDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLWEPEETVVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAESGL

PTMs - Q08881 基板として

Site PTM Type Enzyme
S22 Phosphorylation
Y40 Phosphorylation
K47 Ubiquitination
T50 Phosphorylation
K52 Ubiquitination
K61 Ubiquitination
K67 Ubiquitination
Y90 Phosphorylation
K107 Ubiquitination
S115 Phosphorylation
K119 Ubiquitination
Y120 Phosphorylation
K129 Ubiquitination
K138 Ubiquitination
T141 Phosphorylation
Y146 Phosphorylation
K150 Ubiquitination
K154 Ubiquitination
K155 Ubiquitination
T160 Phosphorylation
Y180 Phosphorylation P42680 (TEC) , Q06187 (BTK) , Q08881 (ITK)
Y182 Phosphorylation
T184 Phosphorylation
Y198 Phosphorylation
S203 Phosphorylation
Y220 Phosphorylation
S223 Phosphorylation
S224 Phosphorylation
Y225 Phosphorylation
K229 Ubiquitination
T236 Phosphorylation
Y237 Phosphorylation
K242 Ubiquitination
K248 Ubiquitination
K251 Ubiquitination
K258 Ubiquitination
Y273 Phosphorylation
T274 Phosphorylation
S276 Phosphorylation
K291 Ubiquitination
K296 Acetylation
K296 Ubiquitination
K303 Ubiquitination
Y336 Phosphorylation
K344 Ubiquitination
Y353 Phosphorylation
K391 Ubiquitination
K412 Ubiquitination
K497 Ubiquitination
S499 Phosphorylation
T504 Phosphorylation
Y512 Phosphorylation P06239 (LCK)
T513 Phosphorylation
S514 Phosphorylation
S515 Phosphorylation
T516 Phosphorylation
T518 Phosphorylation
K519 Ubiquitination
K523 Ubiquitination
S565 Phosphorylation Q08881 (ITK)
T585 Phosphorylation
Y588 Phosphorylation

PTMs - Q08881 酵素として

Substrate Site Source
O43561 (LAT) Y200 Uniprot
O43561 (LAT) Y220 Uniprot
P10747-1 (CD28) Y191 Uniprot
P10747-1 (CD28) Y206 Uniprot
P10747-1 (CD28) Y209 Uniprot
P10747 (CD28) Y218 Uniprot
P19174 (PLCG1) Y775 Uniprot
P19174 (PLCG1) Y783 Uniprot
P42680 (TEC) Y206 Uniprot
P51813 (BMX) Y216 Uniprot
P51813 (BMX) Y224 Uniprot
Q06187 (BTK) Y223 Uniprot
Q08881 (ITK) Y180 Uniprot
Q08881 (ITK) S565 Uniprot
Q13094 (LCP2) Y173 Uniprot
Q8TDQ0 (HAVCR2) Y265 Uniprot
Q96LC7 (SIGLEC10) Y597 Uniprot
Q96LC7 (SIGLEC10) Y667 Uniprot
Q9H4E7 (DEF6) Y210 Uniprot
Q9H4E7 (DEF6) Y222 Uniprot

研究背景

機能:

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).

PTMs:

Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain.

Ubiquitinated.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

T-cell lines and natural killer cell lines.

サブユニット構造:

Homooligomerizes; this association negatively regulates kinase activity (By similarity). Interacts with PPIA/CYPA; this interaction regulates TCR signal strength via a proline-directed conformational switch in ITK. Interacts with THEMIS (By similarity). Interacts with FASLG. Interacts with VAV1; this interaction is important for VAV1 localization and TCR-induced actin polarization. Interacts with TBX21 (By similarity).

タンパク質ファミリー:

The N-terminal PH domain allows ITK to be recruited to the plasma membrane by an activated PI3 kinase. This domain contains also a proline-rich region (PRR). The adjoining domain is a SH3 domain, which binds to PRR (from itself or from other proteins). Next, a SH2 domain is required for binding tyrosine-phosphorylated substrates. In the C-terminal region, the kinase domain is required for tyrosine phosphorylation.

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

研究領域

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

参考文献

1). TBK1 Facilitates GLUT1-Dependent Glucose Consumption by suppressing mTORC1 Signaling in Colorectal Cancer Progression. International Journal of Biological Sciences, 2023 (PubMed: 35637944) [IF=9.2]

2). Dioscin Alleviates Periodontitis by Inhibiting NLRP3 Inflammasome Activation via Regulation of K+ Homeostasis and Mitochondrial Function. International journal of biological sciences, 2024 (PubMed: 38385066) [IF=9.2]

3). Extracellular vesicles delivering nuclear factor I/C for hard tissue engineering: Treatment of apical periodontitis and dentin regeneration. Journal of Tissue Engineering, 2022 (PubMed: 35321254) [IF=8.2]

4). Salidroside alleviates dexamethasone-induced inhibition of bone formation via transforming growth factor-beta/Smad2/3 signaling pathway. Phytotherapy Research, 2023 (PubMed: 36567454) [IF=7.2]

5). Pyrroloquinoline quinone ameliorates liver injury in mice induced by cyclophosphamide. Environmental Science and Pollution Research, 2022 (PubMed: 34997497) [IF=5.8]

6). Activation of Cannabinoid Type 2 Receptor in Microglia Reduces Neuroinflammation through Inhibiting Aerobic Glycolysis to Relieve Hypertension. Biomolecules, 2024 (PubMed: 38540753) [IF=5.5]

7). Tripartite motif containing 33 demonstrated anticancer effect by degrading c‑Myc: Limitation of glutamine metabolism and proliferation in endometrial carcinoma cells. International journal of oncology, 2023 (PubMed: 37859625) [IF=5.2]

8). Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) alleviate excessive autophagy of ovarian granular cells through VEGFA/PI3K/AKT/mTOR pathway in premature ovarian failure rat model. Journal of Ovarian Research, 2023 (PubMed: 37777781) [IF=4.0]

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