ITK Antibody - #AF0003
製品: | ITK Antibody |
カタログ: | AF0003 |
タンパク質の説明: | Rabbit polyclonal antibody to ITK |
アプリケーション: | ELISA(peptide) |
反応性: | Human, Mouse, Rat |
分子量: | 68kDa; 72kD(Calculated). |
ユニプロット: | Q08881 |
RRID: | AB_2846781 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0003, RRID:AB_2846781.
折りたたみ/展開
EMT; Homolog of mouse T cell itk/tsk; IL 2 inducible T cell kinase; IL2 inducible T cell kinase; Interleukin 2 inducible T cell kinase; Itk; ITK_HUMAN; Kinase EMT; LPFS1; LYK; MGC126257; MGC126258; PSCTK 2; PSCTK2; T cell specific kinase; T-cell-specific kinase; TSK; Tyrosine protein kinase; Tyrosine protein kinase ITK/TSK; Tyrosine protein kinase Lyk; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk;
免疫原
A synthesized peptide derived from human ITK
- Q08881 ITK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDRESRQRWVLALKEETRNNNSLVPKYHPNFWMDGKWRCCSQLEKLATGCAQYDPTKNASKKPLPPTPEDNRRPLWEPEETVVIALYDYQTNDPQELALRRNEEYCLLDSSEIHWWRVQDRNGHEGYVPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTAGTYTVSVFTKAVVSENNPCIKHYHIKETNDNPKRYYVAEKYVFDSIPLLINYHQHNGGGLVTRLRYPVCFGRQKAPVTAGLRYGKWVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAESGL
PTMs - Q08881 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S22 | Phosphorylation | Uniprot | |
Y40 | Phosphorylation | Uniprot | |
K47 | Ubiquitination | Uniprot | |
T50 | Phosphorylation | Uniprot | |
K52 | Ubiquitination | Uniprot | |
K61 | Ubiquitination | Uniprot | |
K67 | Ubiquitination | Uniprot | |
Y90 | Phosphorylation | Uniprot | |
K107 | Ubiquitination | Uniprot | |
S115 | Phosphorylation | Uniprot | |
K119 | Ubiquitination | Uniprot | |
Y120 | Phosphorylation | Uniprot | |
K129 | Ubiquitination | Uniprot | |
K138 | Ubiquitination | Uniprot | |
T141 | Phosphorylation | Uniprot | |
Y146 | Phosphorylation | Uniprot | |
K150 | Ubiquitination | Uniprot | |
K154 | Ubiquitination | Uniprot | |
K155 | Ubiquitination | Uniprot | |
T160 | Phosphorylation | Uniprot | |
Y180 | Phosphorylation | P42680 (TEC) , Q06187 (BTK) , Q08881 (ITK) | Uniprot |
Y182 | Phosphorylation | Uniprot | |
T184 | Phosphorylation | Uniprot | |
Y198 | Phosphorylation | Uniprot | |
S203 | Phosphorylation | Uniprot | |
Y220 | Phosphorylation | Uniprot | |
S223 | Phosphorylation | Uniprot | |
S224 | Phosphorylation | Uniprot | |
Y225 | Phosphorylation | Uniprot | |
K229 | Ubiquitination | Uniprot | |
T236 | Phosphorylation | Uniprot | |
Y237 | Phosphorylation | Uniprot | |
K242 | Ubiquitination | Uniprot | |
K248 | Ubiquitination | Uniprot | |
K251 | Ubiquitination | Uniprot | |
K258 | Ubiquitination | Uniprot | |
Y273 | Phosphorylation | Uniprot | |
T274 | Phosphorylation | Uniprot | |
S276 | Phosphorylation | Uniprot | |
K291 | Ubiquitination | Uniprot | |
K296 | Acetylation | Uniprot | |
K296 | Ubiquitination | Uniprot | |
K303 | Ubiquitination | Uniprot | |
Y336 | Phosphorylation | Uniprot | |
K344 | Ubiquitination | Uniprot | |
Y353 | Phosphorylation | Uniprot | |
K391 | Ubiquitination | Uniprot | |
K412 | Ubiquitination | Uniprot | |
K497 | Ubiquitination | Uniprot | |
S499 | Phosphorylation | Uniprot | |
T504 | Phosphorylation | Uniprot | |
Y512 | Phosphorylation | P06239 (LCK) | Uniprot |
T513 | Phosphorylation | Uniprot | |
S514 | Phosphorylation | Uniprot | |
S515 | Phosphorylation | Uniprot | |
T516 | Phosphorylation | Uniprot | |
T518 | Phosphorylation | Uniprot | |
K519 | Ubiquitination | Uniprot | |
K523 | Ubiquitination | Uniprot | |
S565 | Phosphorylation | Q08881 (ITK) | Uniprot |
T585 | Phosphorylation | Uniprot | |
Y588 | Phosphorylation | Uniprot |
PTMs - Q08881 酵素として
Substrate | Site | Source |
---|---|---|
O43561 (LAT) | Y200 | Uniprot |
O43561 (LAT) | Y220 | Uniprot |
P10747-1 (CD28) | Y191 | Uniprot |
P10747-1 (CD28) | Y206 | Uniprot |
P10747-1 (CD28) | Y209 | Uniprot |
P10747 (CD28) | Y218 | Uniprot |
P19174 (PLCG1) | Y775 | Uniprot |
P19174 (PLCG1) | Y783 | Uniprot |
P42680 (TEC) | Y206 | Uniprot |
P51813 (BMX) | Y216 | Uniprot |
P51813 (BMX) | Y224 | Uniprot |
Q06187 (BTK) | Y223 | Uniprot |
Q08881 (ITK) | Y180 | Uniprot |
Q08881 (ITK) | S565 | Uniprot |
Q13094 (LCP2) | Y173 | Uniprot |
Q8TDQ0 (HAVCR2) | Y265 | Uniprot |
Q96LC7 (SIGLEC10) | Y597 | Uniprot |
Q96LC7 (SIGLEC10) | Y667 | Uniprot |
Q9H4E7 (DEF6) | Y210 | Uniprot |
Q9H4E7 (DEF6) | Y222 | Uniprot |
研究背景
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).
Phosphorylated at Tyr-512 in the activation loop of the kinase domain by LCK. Subsequent autophosphorylation at Tyr-180 leads to the kinase activation. The autophosphorylated Tyr-180 lies within the substrate binding sequence of the SH3 domain.
Ubiquitinated.
Cytoplasm. Nucleus.
Note: Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation.
T-cell lines and natural killer cell lines.
Homooligomerizes; this association negatively regulates kinase activity (By similarity). Interacts with PPIA/CYPA; this interaction regulates TCR signal strength via a proline-directed conformational switch in ITK. Interacts with THEMIS (By similarity). Interacts with FASLG. Interacts with VAV1; this interaction is important for VAV1 localization and TCR-induced actin polarization. Interacts with TBX21 (By similarity).
The N-terminal PH domain allows ITK to be recruited to the plasma membrane by an activated PI3 kinase. This domain contains also a proline-rich region (PRR). The adjoining domain is a SH3 domain, which binds to PRR (from itself or from other proteins). Next, a SH2 domain is required for binding tyrosine-phosphorylated substrates. In the C-terminal region, the kinase domain is required for tyrosine phosphorylation.
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
研究領域
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
参考文献
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