EPS15 Antibody - #AF0005

製品: EPS15 Antibody
カタログ: AF0005
タンパク質の説明: Rabbit polyclonal antibody to EPS15
アプリケーション: WB
反応性: Human, Mouse, Rat
分子量: 100~150kDa; 99kD(Calculated).
ユニプロット: P42566
RRID: AB_2846782

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
EPS15 Antibody detects endogenous levels of total EPS15.
RRID:
AB_2846782
引用形式: Affinity Biosciences Cat# AF0005, RRID:AB_2846782.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AF 1P; AF 1p protein; AF1P; ALL1 fused gene from chromosome 1; Epidermal growth factor receptor pathway substrate 15; Epidermal growth factor receptor substrate 15; EPS 15; MLLT 5; MLLT5; Protein Eps 15; Protein Eps15;

免疫原

免疫原:

A synthesized peptide derived from human EPS15.

Uniprot:

P42566(EPS15_HUMAN) >>Visit HPA database.

遺伝子(ID):
EPS15(2060)
発現特異性:
P42566 EPS15_HUMAN:

Ubiquitously expressed.

タンパク質の説明:
This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
タンパク質配列:
MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLVACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKLPVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNIIGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQHLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPARSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA

PTMs - P42566 基板として

Site PTM Type Enzyme
A2 Acetylation
S8 Phosphorylation
T106 Phosphorylation
S107 Phosphorylation
S108 Phosphorylation
S140 Phosphorylation
S221 Phosphorylation
K227 Methylation
K227 Ubiquitination
K234 Methylation
K234 Ubiquitination
S244 Phosphorylation
T304 Phosphorylation
S315 Phosphorylation
K318 Ubiquitination
S323 Phosphorylation
S324 Phosphorylation
K333 Ubiquitination
K357 Acetylation
K359 Acetylation
K386 Ubiquitination
K391 Ubiquitination
K413 Ubiquitination
S435 Phosphorylation
Y443 Phosphorylation
K449 Ubiquitination
S455 Phosphorylation
S467 Phosphorylation
S470 Phosphorylation
K472 Ubiquitination
S485 Phosphorylation
K495 Ubiquitination
S551 Phosphorylation
S558 Phosphorylation
S562 Phosphorylation
S563 Phosphorylation
S587 Phosphorylation
S595 Phosphorylation
S605 Phosphorylation
S606 Phosphorylation
K632 Ubiquitination
K638 Ubiquitination
K648 Ubiquitination
S655 Phosphorylation
T663 Phosphorylation
S680 Phosphorylation
S681 Phosphorylation
S719 Phosphorylation
S741 Phosphorylation
S746 Phosphorylation
K754 Ubiquitination
K763 Ubiquitination
K774 Ubiquitination
T777 Phosphorylation
T779 Phosphorylation
K788 Acetylation
K788 Ubiquitination
S790 Phosphorylation
K793 Ubiquitination
S796 Phosphorylation Q16539 (MAPK14)
K801 Ubiquitination
S814 Phosphorylation
K816 Ubiquitination
K818 Ubiquitination
K836 Ubiquitination
Y849 Phosphorylation P00533 (EGFR)
Y850 Phosphorylation
S851 Phosphorylation
K860 Ubiquitination
K890 Ubiquitination

研究背景

機能:

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

PTMs:

Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.

Ubiquitinated.

細胞の位置付け:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Clathrin-coated pit.
Note: Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.

Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Colocalizes with HGS on bilayered clathrin coats on endosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed.

サブユニット構造:

Interacts with SGIP1. Interacts with HGS; the interaction bridges the interaction of STAM or STAM2 with EPS15. Isoform 2 interacts with HGS and AP2A2. Part of a complex at least composed of EPS15, HGS, and either STAM or STAM2. Binds AP2A2. Interacts with AP2B1; clathrin competes with EPS15. Binds STON2 and EPN1. Interacts (via its SH3-binding sites) with CRK. Interacts with SH3BP4/TTP. Interacts with ERBB2. Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts (via UIM repeats) with CORO7 (when ubiquitinated at 'Lys-472'). Interacts (via UIM domains) with UBQLN1 (via ubiquitin-like domain) and can interact with both the ubiquitinated and the non-ubiquitinated forms of UBQLN1. Interacts with UBQLN2 (By similarity).

(Microbial infection) Interacts with vaccinia virus protein A36.

タンパク質ファミリー:

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.

The UIM (ubiquitin-interacting motif) repeats specifically bind 'Lys-33'-linked ubiquitin.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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