FANCG Antibody - #AF0006

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製品: FANCG Antibody
カタログ: AF0006
タンパク質の説明: Rabbit polyclonal antibody to FANCG
アプリケーション: WB
反応性: Human, Mouse, Rat
分子量: 69kDa; 69kD(Calculated).
ユニプロット: O15287
RRID: AB_2846783

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
FANCG Antibody detects endogenous levels of total FANCG.
RRID:
AB_2846783
引用形式: Affinity Biosciences Cat# AF0006, RRID:AB_2846783.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DNA repair protein XRCC9; FAG; FANCG; FANCG_HUMAN; Fanconi anaemia complementation group G; Fanconi anemia group G protein; Protein FACG; X ray repair, complementing defective, in Chinese hamster cells 9; X-ray repair, complementing defective, in Chinese hamster, 9; XRCC9;

免疫原

免疫原:

A synthesized peptide derived from human FANCG.

Uniprot:

O15287(FANCG_HUMAN) >>Visit HPA database.

遺伝子(ID):
FANCG(2189)
発現特異性:
O15287 FANCG_HUMAN:

Highly expressed in testis and thymus. Found in lymphoblasts.

タンパク質の説明:
FANCG DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene. Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. Note: This description may include information from UniProtKB.
タンパク質配列:
MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGLTLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLELTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLETQEQQGPRLEQGLRELWDSVLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHILASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPPPSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEELLSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSATHLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEEKEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWVASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKRLDRRDEATALWWRLEAQTKGSHEDALWSLPLYLESYLSWIRPSDRDAFLEEFRTSLPKSCDL

PTMs - O15287 基板として

Site PTM Type Enzyme
S7 Phosphorylation
K28 Ubiquitination
K182 Ubiquitination
K258 Ubiquitination
K347 Ubiquitination
S383 Phosphorylation P06493 (CDK1)
S387 Phosphorylation P06493 (CDK1)
K430 Ubiquitination
T487 Phosphorylation P06493 (CDK1)
K491 Ubiquitination
K504 Ubiquitination
T557 Phosphorylation
K559 Ubiquitination
K618 Ubiquitination

研究背景

機能:

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.

細胞の位置付け:

Nucleus. Cytoplasm.
Note: The major form is nuclear. The minor form is cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in testis and thymus. Found in lymphoblasts.

サブユニット構造:

Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCA and FANCL, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. The complex with FANCC and FANCG may also include EIF2AK2 and HSP70. When phosphorylated at Ser-7, forms a complex with BRCA2, FANCD2 and XRCC3.

研究領域

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

参考文献

1). TBK1 Facilitates GLUT1-Dependent Glucose Consumption by suppressing mTORC1 Signaling in Colorectal Cancer Progression. International Journal of Biological Sciences, 2023 (PubMed: 35637944) [IF=9.2]
2). Single xenotransplant of rat brown adipose tissue prolonged the ovarian lifespan of aging mice by improving follicle survival. Aging Cell, 2019 (PubMed: 31389140) [IF=7.8]
3). FTO Positively Regulates Odontoblastic Differentiation via SMOC2 in Human Stem Cells from the Apical Papilla under Inflammatory Microenvironment. International journal of molecular sciences, 2024 (PubMed: 38612855) [IF=5.6]

Application: WB    Species: Human    Sample: hSCAPs

Figure 3 hSCAPs’ odontoblastic differentiation ability and FTO expression under different conditions. (a–c) ALP staining at 3 and 7 days showed decreased ALP activity, and ARS staining at day 14 indicated reduced mineralized nodules in the OM+LPS group compared to the OM group. (d) qRT-PCR revealed elevated mRNA levels of DSPP, DMP1, and COL1 in the OM group while showing decreased levels in the OM+LPS group. (e,f) Western blotting showed increased protein expression of DSPP, DMP1, and COL1 in the OM group, contrasting with decreased expression in the OM+LPS group. (g–i) qRT-PCR and Western blotting showed elevated FTO expression in OM, with reduced expression observed in OM+LPS. Control, growth medium (GM); LPS, GM with 1 µg/mL LPS; OM, odontoblastic induction medium; OM+LPS, OM with 1 µg/mL LPS; hSCAPs, human stem cells from the apical papilla; FTO, fat mass and obesity-associated protein; LPS, lipopolysaccharide; ALP, alkaline phosphatase; ARS, alizarin red S; qRT-PCR, Quantitative Real-Time Polymerase Chain Reaction; DSPP, dentin sialophosphoprotein; DMP1, dentin matrix protein-1; COL1, collagen I.
4). NLRC4-mediated pyroptosis was involved in coagulation disorders of acute pancreatitis. The journal of gene medicine, 2024 (PubMed: 38571451) [IF=3.5]

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