ACSS1 Antibody - #AF1001
| 製品: | ACSS1 Antibody |
| カタログ: | AF1001 |
| タンパク質の説明: | Rabbit polyclonal antibody to ACSS1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 70 kDa; 75kD(Calculated). |
| ユニプロット: | Q9NUB1 |
| RRID: | AB_2846798 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF1001, RRID:AB_2846798.
折りたたみ／展開
ACAS2L; AceCS2; AceCS2L; Acetate CoA ligase 2; Acetate--CoA ligase 2; Acetyl CoA synthetase 2; Acetyl Coenzyme A synthetase 2 (AMP forming) like; Acetyl coenzyme A synthetase 2 like, mitochondrial; Acetyl-CoA synthetase 2; Acetyl-coenzyme A synthetase 2-like, mitochondrial; ACS2L_HUMAN; Acss1; Acyl CoA synthetase short chain family member 1; Acyl-CoA synthetase short-chain family member 1; FLJ45659; KIAA1846; MGC33843;
免疫原
A synthesized peptide derived from human ACSS1 around the acetylation site of Lys642.
- Q9NUB1 ACS2L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAARTLGRGVGRLLGSLRGLSGQPARPPCGVSAPRRAASGPSGSAPAVAAAAAQPGSYPALSAQAAREPAAFWGPLARDTLVWDTPYHTVWDCDFSTGKIGWFLGGQLNVSVNCLDQHVRKSPESVALIWERDEPGTEVRITYRELLETTCRLANTLKRHGVHRGDRVAIYMPVSPLAVAAMLACARIGAVHTVIFAGFSAESLAGRINDAKCKVVITFNQGLRGGRVVELKKIVDEAVKHCPTVQHVLVAHRTDNKVHMGDLDVPLEQEMAKEDPVCAPESMGSEDMLFMLYTSGSTGMPKGIVHTQAGYLLYAALTHKLVFDHQPGDIFGCVADIGWITGHSYVVYGPLCNGATSVLFESTPVYPNAGRYWETVERLKINQFYGAPTAVRLLLKYGDAWVKKYDRSSLRTLGSVGEPINCEAWEWLHRVVGDSRCTLVDTWWQTETGGICIAPRPSEEGAEILPAMAMRPFFGIVPVLMDEKGSVVEGSNVSGALCISQAWPGMARTIYGDHQRFVDAYFKAYPGYYFTGDGAYRTEGGYYQITGRMDDVINISGHRLGTAEIEDAIADHPAVPESAVIGYPHDIKGEAAFAFIVVKDSAGDSDVVVQELKSMVATKIAKYAVPDEILVVKRLPKTRSGKVMRRLLRKIITSEAQELGDTTTLEDPSIIAEILSVYQKCKDKQAAAK
PTMs - Q9NUB1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| K396 | Acetylation | Uniprot | |
| Y521 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| Y542 | Phosphorylation | Uniprot | |
| K633 | Ubiquitination | Uniprot | |
| K642 | Acetylation | Uniprot | |
| Y678 | Phosphorylation | Uniprot |
研究背景
Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity (By similarity). Provides acetyl-CoA that is utilized mainly for oxidation under ketogenic conditions (By similarity). Involved in thermogenesis under ketogenic conditions, using acetate as a vital fuel when carbohydrate availability is insufficient (By similarity).
Reversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylase SIRT3.
Mitochondrion matrix.
Interacts with SIRT3.
Belongs to the ATP-dependent AMP-binding enzyme family.
研究領域
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.