Phospho-MAP3K4 (Ser499) Antibody - #AF3502
| 製品: | Phospho-MAP3K4 (Ser499) Antibody |
| カタログ: | AF3502 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-MAP3K4 (Ser499) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human |
| 分子量: | 182kD(Calculated). |
| ユニプロット: | Q9Y6R4 |
| RRID: | AB_2846816 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3502, RRID:AB_2846816.
折りたたみ／展開
dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4); FLJ42439; KIAA0213; M3K4_HUMAN; MAP three kinase 1; MAP/ERK kinase kinase 4; MAP3K4; MAPK/ERK kinase kinase 4; MAPKKK4; MEK kinase 4; MEKK 4; MEKK4; Mitogen-activated protein kinase kinase kinase 4; MTK1; predicted protein of HQ0412; PRO0412; RP3-473J16.4; SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of;
免疫原
A synthesized peptide derived from human MAP3K4 around the phosphorylation site of Ser499.
Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.
- Q9Y6R4 M3K4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MREAAAALVPPPAFAVTPAAAMEEPPPPPPPPPPPPEPETESEPECCLAARQEGTLGDSACKSPESDLEDFSDETNTENLYGTSPPSTPRQMKRMSTKHQRNNVGRPASRSNLKEKMNAPNQPPHKDTGKTVENVEEYSYKQEKKIRAALRTTERDRKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIARPARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSGFWLNRSNELIWLELQAWHAGRTINDQDFFLYTARQAIPDIINEILTFKVDYGSFAFVRDRAGFNGTSVEGQCKATPGTKIVGYSTHHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEYEGDDTEGELKELESSTDESEEEQISDPRVPEIRQPIDNSFDIQSRDCISKKLERLESEDDSLGWGAPDWSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKNDRPVEFSEFPDPMWGSDYVQLSRTPPSSEEKCSAVSWEELKAMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLQEVLEDLEKPDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSSASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFRLSAPVRDLLDVLKSKQYVKVQIPGLENLQMFVPDTLAEEKSIILQLLNAAAGKDCSKDSDDVLIDAYLLLTKHGDRARDSEDSWGTWEAQPVKVVPQVETVDTLRSMQVDNLLLVVMQSAHLTIQRKAFQQSIEGLMTLCQEQTSSQPVIAKALQQLKNDALELCNRISNAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPMKVPRCHSDPPNPHLIIPTPEGFSTRSMPSDARSHGSPAAAAAAAAAAVAASRPSPSGGDSVLPKSISSAHDTRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPRGDSSGSTRRSWELRTLISQSKDTASKLGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITIAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESDPKMRWTASQLLDHSFVKVCTDEE
PTMs - Q9Y6R4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S63 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| Y81 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K98 | Acetylation | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S431 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| T447 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| Y582 | Phosphorylation | Uniprot | |
| S798 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Uniprot | |
| K890 | Acetylation | Uniprot | |
| S979 | Phosphorylation | Uniprot | |
| S1068 | Phosphorylation | Uniprot | |
| Y1076 | Phosphorylation | Uniprot | |
| S1135 | Phosphorylation | Uniprot | |
| S1148 | Phosphorylation | Uniprot | |
| S1159 | Phosphorylation | Uniprot | |
| S1178 | Phosphorylation | Uniprot | |
| S1252 | Phosphorylation | Uniprot | |
| S1268 | Phosphorylation | Uniprot | |
| S1270 | Phosphorylation | Uniprot | |
| T1271 | Phosphorylation | Uniprot | |
| S1274 | Phosphorylation | Uniprot | |
| S1282 | Phosphorylation | Uniprot | |
| Y1405 | Phosphorylation | Uniprot | |
| T1494 | Phosphorylation | Q9Y6R4 (MAP3K4) | Uniprot |
| T1505 | Phosphorylation | Uniprot | |
| Y1548 | Phosphorylation | Uniprot | |
| Y1556 | Phosphorylation | Uniprot | |
| K1575 | Ubiquitination | Uniprot | |
| S1579 | Phosphorylation | Uniprot | |
| S1584 | Phosphorylation | Uniprot |
研究背景
Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Cytoplasm>Perinuclear region.
Note: Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.
Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.
Monomer and homodimer. Homodimerization enhances kinase activity. Interacts with TRAF4; this promotes homodimerization. Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with AXIN1 and DIXDC1; interaction with DIXDC1 prevents interaction with AXIN1. Interacts with GADD45 and MAP2K6. Interacts with ZFP36; this interaction enhances the association with SH3KBP1/CIN85. Interacts with SH3KBP1; this interaction enhances the association with ZFP36. Interacts with CDC42 (By similarity).
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
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