Phospho-ATG4B (Ser316) Antibody - #AF3505
| 製品: | Phospho-ATG4B (Ser316) Antibody |
| カタログ: | AF3505 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-ATG4B (Ser316) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse |
| 分子量: | 44kD(Calculated). |
| ユニプロット: | Q9Y4P1 |
| RRID: | AB_2846819 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3505, RRID:AB_2846819.
折りたたみ／展開
APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; ATG4B_HUMAN; AUT like 1 cysteine endopeptidase; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagin-1; Autophagy related 4B cysteine peptidase; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353;
免疫原
A synthesized peptide derived from human ATG4B around the phosphorylation site of Ser316.
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
- Q9Y4P1 ATG4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL
PTMs - Q9Y4P1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| Y123 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot |
研究背景
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Cytoplasm.
Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.
Belongs to the peptidase C54 family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
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