Phospho-DYRK1A/B (Tyr319/Tyr271) Antibody - #AF3507

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製品: Phospho-DYRK1A/B (Tyr319/Tyr271) Antibody
カタログ: AF3507
タンパク質の説明: Rabbit polyclonal antibody to Phospho-DYRK1A/B (Tyr319/Tyr271)
アプリケーション: IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 86kD,69kD(Calculated).
ユニプロット: Q13627 | Q9Y463
RRID: AB_2846821

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-DYRK1A/B (Tyr319/Tyr271) Antibody detects endogenous levels of DYRK1A/B only when phosphorylated at Tyr319/271.
RRID:
AB_2846821
引用形式: Affinity Biosciences Cat# AF3507, RRID:AB_2846821.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Dual specificity tyrosine phosphorylation regulated kinase; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1A; Dual specificity tyrosine phosphorylation regulated kinase 1; Dual specificity tyrosine phosphorylation regulated kinase 1A; Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Dual specificity YAK 1 related kinase; Dual specificity YAK1 related kinase; Dual specificity YAK1-related kinase; DYR1A_HUMAN; DYRK 1; DYRK 1A; DYRK; DYRK1; Dyrk1a; DYRKA; hMNB; HP 86; HP86; Minibrain (Drosophila) homolog; Minibrain homolog; Minibrain, Drosophila, homolog of; MNB; MNB protein kinase; Mnb protein kinase homolog hp86; MNB protein kinase, Serine/threonine-specific; MNB/DYRK protein kinase; MNBH; MRD7; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; Protein kinase minibrain homolog; Serine/threonine kinase MNB; AOMS3; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B; Dual specificity tyrosine phosphorylation regulated kinase 1B; Dual spec

免疫原

免疫原:

A synthesized peptide derived from human DYRK1A/B around the phosphorylation site of Tyr319/271.

Uniprot:

Q13627(DYR1A_HUMAN) >>Visit HPA database.

Q9Y463(DYR1B_HUMAN) >>Visit HPA database.

遺伝子(ID):
DYRK1A(1859) | DYRK1B(9149)
発現特異性:
Q13627 DYR1A_HUMAN:

Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.

Q9Y463 DYR1B_HUMAN:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

タンパク質配列:
MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS

MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS

PTMs - Q13627/Q9Y463 基板として

Site PTM Type Enzyme
S49 Phosphorylation
K57 Acetylation
Y63 Phosphorylation
Y64 Phosphorylation
Y111 Phosphorylation
K119 Ubiquitination
K127 Ubiquitination
K256 Ubiquitination
Y271 Phosphorylation Q9Y463 (DYRK1B)
Y273 Phosphorylation Q9Y463 (DYRK1B)
S276 Phosphorylation
S421 Phosphorylation Q9Y463 (DYRK1B) , P28482 (MAPK1)
Y485 Phosphorylation
S486 Phosphorylation
S576 Phosphorylation
Site PTM Type Enzyme
K11 Ubiquitination
S14 Phosphorylation
S22 Phosphorylation
S97 Phosphorylation
Y104 Phosphorylation Q13627 (DYRK1A)
K105 Acetylation
Y111 Phosphorylation Q13627 (DYRK1A)
Y112 Phosphorylation
S127 Phosphorylation
K134 Ubiquitination
Y136 Phosphorylation
Y140 Phosphorylation Q13627 (DYRK1A)
Y145 Phosphorylation
Y147 Phosphorylation
K150 Ubiquitination
Y159 Phosphorylation Q13627 (DYRK1A)
K167 Ubiquitination
Y177 Phosphorylation Q13627 (DYRK1A)
K304 Ubiquitination
S310 Phosphorylation Q13627 (DYRK1A)
Y319 Phosphorylation Q13627 (DYRK1A)
Y321 Phosphorylation Q13627 (DYRK1A)
S324 Phosphorylation
S329 Phosphorylation
T402 Phosphorylation Q13627 (DYRK1A)
Y449 Phosphorylation Q13627 (DYRK1A)
K453 Ubiquitination
T466 Phosphorylation
S529 Phosphorylation Q13627 (DYRK1A)
T532 Phosphorylation
S538 Phosphorylation
T543 Phosphorylation
T553 Phosphorylation
S555 Phosphorylation
Y705 Phosphorylation
S706 Phosphorylation
S748 Phosphorylation
S758 Phosphorylation
S762 Phosphorylation

PTMs - Q13627/Q9Y463 酵素として

Substrate Site Source
O75533 (SF3B1) T434 Uniprot
P20823 (HNF1A) S249 Uniprot
P24385 (CCND1) T286 Uniprot
P24385 (CCND1) T288 Uniprot
P38936 (CDKN1A) S153 Uniprot
P46527 (CDKN1B) S10 Uniprot
Q02363 (ID2) T27 Uniprot
Q99801 (NKX3-1) S185 Uniprot
Q9UQL6 (HDAC5) S279 Uniprot
Q9Y463 (DYRK1B) Y271 Uniprot
Q9Y463 (DYRK1B) Y273 Uniprot
Q9Y463 (DYRK1B) S421 Uniprot
Substrate Site Source
O00401 (WASL) T199 Uniprot
O00401 (WASL) T205 Uniprot
O00401 (WASL) T262 Uniprot
O43524 (FOXO3) S330 Uniprot
O43597 (SPRY2) T75 Uniprot
O75533 (SF3B1) T273 Uniprot
O75533 (SF3B1) T434 Uniprot
P01106 (MYC) S62 Uniprot
P04637 (TP53) S15 Uniprot
P10636-8 (MAPT) S199 Uniprot
P10636-8 (MAPT) S202 Uniprot
P10636-8 (MAPT) T205 Uniprot
P10636-8 (MAPT) T212 Uniprot
P10636-8 (MAPT) T217 Uniprot
P10636 (MAPT) S369 Uniprot
P10636-8 (MAPT) S396 Uniprot
P10636-8 (MAPT) S404 Uniprot
P10636-8 (MAPT) S422 Uniprot
P10636 (MAPT) S519 Uniprot
P10636 (MAPT) T529 Uniprot
P10636 (MAPT) S721 Uniprot
P16220 (CREB1) S133 Uniprot
P24385 (CCND1) T286 Uniprot
P24928 (POLR2A) S1616 Uniprot
P24928 (POLR2A) S1619 Uniprot
P37840 (SNCA) S87 Uniprot
P46821 (MAP1B) S1400 Uniprot
P49418 (AMPH) S262 Uniprot
P49418 (AMPH) S272 Uniprot
P49418 (AMPH) S276 Uniprot
P49418 (AMPH) S285 Uniprot
P49418 (AMPH) S293 Uniprot
P49418 (AMPH) S295 Uniprot
P49418 (AMPH) T310 Uniprot
P49418 (AMPH) T312 Uniprot
P49768 (PSEN1) T354 Uniprot
P53805 (RCAN1) S167 Uniprot
P53805 (RCAN1) T247 Uniprot
P55211 (CASP9) T125 Uniprot
P61764 (STXBP1) T479 Uniprot
Q05193 (DNM1) S795 Uniprot
Q05193 (DNM1) S857 Uniprot
Q07955 (SRSF1) S227 Uniprot
Q07955 (SRSF1) S234 Uniprot
Q07955 (SRSF1) S238 Uniprot
Q12778 (FOXO1) S329 Uniprot
Q13627 (DYRK1A) Y104 Uniprot
Q13627 (DYRK1A) Y111 Uniprot
Q13627 (DYRK1A) Y140 Uniprot
Q13627 (DYRK1A) Y159 Uniprot
Q13627 (DYRK1A) Y177 Uniprot
Q13627 (DYRK1A) S310 Uniprot
Q13627 (DYRK1A) Y319 Uniprot
Q13627 (DYRK1A) Y321 Uniprot
Q13627 (DYRK1A) T402 Uniprot
Q13627 (DYRK1A) Y449 Uniprot
Q13627-1 (DYRK1A) S519 Uniprot
Q13627-2 (DYRK1A) S520 Uniprot
Q13627 (DYRK1A) S529 Uniprot
Q49AN0 (CRY2) S558 Uniprot
Q52LA3 (LIN52) S28 Uniprot
Q96EB6 (SIRT1) T530 Uniprot
Q96S94 (CCNL2) S330 Uniprot
Q96S94-1 (CCNL2) S338 Uniprot
Q96S94-1 (CCNL2) S369 Uniprot

研究背景

機能:

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity). Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).

PTMs:

Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).

細胞の位置付け:

Nucleus. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Highest levels in skeletal muscle, testis, fetal lung and fetal kidney.

サブユニット構造:

Interacts RAD54L2/ARIP4 (By similarity). Interacts with CRY2 (By similarity). Interacts with RANBP9. Interacts with WDR68. Interacts with SRSF6. Interacts with SIRT1 (By similarity).

(Microbial infection) Interacts with human adenovirus 5 E1A protein.

タンパク質ファミリー:

The polyhistidine repeats act as targeting signals to nuclear speckles.

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

機能:

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).

PTMs:

Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

サブユニット構造:

Dimer. Interacts with DCOHM, MAP2K3/MKK3, RANBP9 and TCF1/HNF1A. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Interacts with DCAF7.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

参考文献

1). The role of NPY2R/NFATc1/DYRK1A regulatory axis in sebaceous glands for sebum synthesis. Cellular & Molecular Biology Letters, 2023 (PubMed: 37501148) [IF=8.3]

Application: WB    Species: Rat    Sample:

Fig. 5 The activity of NFATc1 induced by DYRK1A in early puberty. A Western blot show the expression of NFATc1 related phosphokinases and their phosphorylation in VS of PND-25 and PND-35. B Western blot show the phosphorylation of NFATc1 affected by DYRK1A inhibitor. C IF show the localization of NFATc1 and phosphorylated NFATc1 in VS of PND-25, PND25 with DYRK1A inhibitor and PND-35 (magnification ×200). The SG specimens from two individuals were used for western blot. The SG specimens from three individuals were used for immunofluorescence

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