Phospho-ASF1A (Ser166) Antibody - #AF3510
| 製品: | Phospho-ASF1A (Ser166) Antibody |
| カタログ: | AF3510 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-ASF1A (Ser166) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 23kD(Calculated). |
| ユニプロット: | Q9Y294 |
| RRID: | AB_2846824 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3510, RRID:AB_2846824.
折りたたみ／展開
Anti silencing function 1A; Anti-silencing function protein 1 homolog A; ASF1 anti silencing function 1 homolog A (S. cerevisiae); ASF1 anti silencing function 1 homolog A; asf1a; ASF1A_HUMAN; CCG1 interacting factor A; CCG1-interacting factor A; CGI 98; CIA; hAsf1; hAsf1a; hCIA; Histone chaperone ASF1A; HSPC146;
免疫原
A synthesized peptide derived from human ASF1A around the phosphorylation site of Ser166.
- Q9Y294 ASF1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKVQVNNVVVLDNPSPFYNPFQFEITFECIEDLSEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNPGLIPDADAVGVTVVLITCTYRGQEFIRVGYYVNNEYTETELRENPPVKPDFSKLQRNILASNPRVTRFHINWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM
PTMs - Q9Y294 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T120 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| T157 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Q86UE8 (TLK2) | Uniprot |
研究背景
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase. TLK2-mediated phosphorylation at Ser-192 prevents proteasome-dependent degradation.
Nucleus.
Ubiquitously expressed.
Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Interacts with CDAN1. Found in a cytosolic complex with CDAN1, ASF1B, IPO4 and histones H3.1 and H4. Interacts with CREBBP.
Belongs to the ASF1 family.
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