Phospho-PIN4 (Tyr122) Antibody - #AF3511

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製品: Phospho-PIN4 (Tyr122) Antibody
カタログ: AF3511
タンパク質の説明: Rabbit polyclonal antibody to Phospho-PIN4 (Tyr122)
アプリケーション: IHC
反応性: Human, Mouse, Rat
分子量: 14kD(Calculated).
ユニプロット: Q9Y237
RRID: AB_2846825

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IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-PIN4 (Tyr122) Antibody detects endogenous levels of PIN4 only when phosphorylated at Tyr122.
RRID:
AB_2846825
引用形式: Affinity Biosciences Cat# AF3511, RRID:AB_2846825.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EPVH; Eukaryotic parvulin homolog; hEPVH; hPar14; hPar17; MGC138486; OTTHUMP00000023527; OTTHUMP00000217483; OTTHUMP00000217485; Par14; Par17; Parvulin 14; Parvulin-14; Parvulin-17; Peptidyl prolyl cis trans isomerase NIMA interacting 4; Peptidyl prolyl cis/trans isomerase EPVH; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4; Peptidyl-prolyl cis-trans isomerase PIN4; Peptidyl-prolyl cis/trans isomerase EPVH; PIN4; PIN4_HUMAN; PPIase Pin4; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin); Rotamase Pin4;

免疫原

免疫原:

A synthesized peptide derived from human PIN4 around the phosphorylation site of Tyr122.

Uniprot:

Q9Y237(PIN4_HUMAN) >>Visit HPA database.

遺伝子(ID):
PIN4(5303)
発現特異性:
Q9Y237 PIN4_HUMAN:

Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.

タンパク質配列:
MPPKGKSGSGKAGKGGAASGSDSADKKAQGPKGGGNAVKVRHILCEKHGKIMEAMEKLKSGMRFNEVAAQYSEDKARQGGDLGWMTRGSMVGPFQEAAFALPVSGMDKPVFTDPPVKTKFGYHIIMVEGRK

研究背景

機能:

Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.

Isoform 2 binds to double-stranded DNA.

PTMs:

Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.

Isoform 2 is sumoylated with SUMO2 and SUMO3.

細胞の位置付け:

Nucleus>Nucleolus. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm.
Note: Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.

Mitochondrion. Mitochondrion matrix.
Note: Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.

サブユニット構造:

Isoform 1 is found in pre-ribosomal ribonucleoprotein (pre-rRNP) complexes.

タンパク質ファミリー:

The PPIase domain enhances mitochondrial targeting.

Belongs to the PpiC/parvulin rotamase family. PIN4 subfamily.

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