Phospho-AurB/C (Thr236/Thr202) Antibody - #AF3512
| 製品: | Phospho-AurB/C (Thr236/Thr202) Antibody |
| カタログ: | AF3512 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-AurB/C (Thr236/Thr202) |
| アプリケーション: | IHC |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 36kD(Calculated). |
| ユニプロット: | Q9UQB9 |
| RRID: | AB_2846826 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3512, RRID:AB_2846826.
折りたたみ／展開
;AURKC; AIE 2; Aie1; AIE2; AIK 3; AIK3; ARK 3; ARK-3; ARK3; Aur C; AurC; Aurkc; AURKC_HUMAN; aurora 3; Aurora C; Aurora kinase C; aurora related kinase 3; Aurora-related kinase 3; Aurora/Ipl1 related kinase 3; Aurora/Ipl1-related kinase 3; Aurora/Ipl1/Eg2 protein 2; EC 2.7.11.1; Serine threonine protein kinase 13; serine/threonine kinase 13 (aurora/IPL1 like); serine/threonine protein kinase aurora C; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase aurora-C; SPGF5; STK13;
免疫原
A synthesized peptide derived from human AurB/C around the phosphorylation site of Thr236/202.
Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cell lines such as Hep-G2, Huh-7 and HeLa. Expression is maximum at M phase.
- Q9UQB9 AURKC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSPRAVVQLGKAQPAGEELATANQTAQQPSSPAMRRLTVDDFEIGRPLGKGKFGNVYLARLKESHFIVALKVLFKSQIEKEGLEHQLRREIEIQAHLQHPNILRLYNYFHDARRVYLILEYAPRGELYKELQKSEKLDEQRTATIIEELADALTYCHDKKVIHRDIKPENLLLGFRGEVKIADFGWSVHTPSLRRKTMCGTLDYLPPEMIEGRTYDEKVDLWCIGVLCYELLVGYPPFESASHSETYRRILKVDVRFPLSMPLGARDLISRLLRYQPLERLPLAQILKHPWVQAHSRRVLPPCAQMAS
PTMs - Q9UQB9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S32 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| Y58 | Phosphorylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot |
PTMs - Q9UQB9 酵素として
| Substrate | Site | Source |
|---|---|---|
| O75410 (TACC1) | S228 | Uniprot |
| P68431 (HIST1H3J) | S11 | Uniprot |
研究背景
Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.
Nucleus. Chromosome. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle.
Note: Distributes in the condensed chromosomes during prophase to metaphase. After entering anaphase, there is a dissociation from separated chromosomes and a redistribution to midzone microtubules, and finally remains in the midbody during cytokinesis.
Isoform 1 and isoform 2 are expressed in testis. Elevated expression levels were seen only in a subset of cancer cell lines such as Hep-G2, Huh-7 and HeLa. Expression is maximum at M phase.
Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; predominantly independent AURKB- and AURKC-containing complexes exist; in the complex interacts directly with BIRC5/survivin and INCENP. Interacts with TACC1.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
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