Phospho-hFANCI (Ser556) Antibody - #AF3524
| 製品: | Phospho-hFANCI (Ser556) Antibody |
| カタログ: | AF3524 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-hFANCI (Ser556) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human |
| 分子量: | 149kD(Calculated). |
| ユニプロット: | Q9NVI1 |
| RRID: | AB_2846838 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3524, RRID:AB_2846838.
折りたたみ／展開
FANCI; FANCI gene; FANCI_HUMAN; Fanconi anemia group I protein; Fanconi anemia, complementation group I; FLJ10719; FLJ14658; KIAA1794; Protein FACI; Protein FANCI;
免疫原
A synthesized peptide derived from human hFANCI around the phosphorylation site of Ser556.
- Q9NVI1 FANCI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMDSLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK
PTMs - Q9NVI1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| K16 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| T62 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| S556 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S559 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S565 | Phosphorylation | Uniprot | |
| Y606 | Phosphorylation | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| K646 | Acetylation | Uniprot | |
| T653 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K780 | Ubiquitination | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| K799 | Ubiquitination | Uniprot | |
| T800 | Phosphorylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| K869 | Ubiquitination | Uniprot | |
| K897 | Ubiquitination | Uniprot | |
| K902 | Ubiquitination | Uniprot | |
| S903 | Phosphorylation | Uniprot | |
| K926 | Ubiquitination | Uniprot | |
| K939 | Ubiquitination | Uniprot | |
| T952 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1000 | Phosphorylation | Uniprot | |
| K1015 | Ubiquitination | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| K1040 | Ubiquitination | Uniprot | |
| K1068 | Ubiquitination | Uniprot | |
| K1105 | Ubiquitination | Uniprot | |
| K1107 | Ubiquitination | Uniprot | |
| S1120 | Phosphorylation | Uniprot | |
| S1121 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K1196 | Ubiquitination | Uniprot | |
| K1199 | Ubiquitination | Uniprot | |
| K1221 | Ubiquitination | Uniprot | |
| K1229 | Ubiquitination | Uniprot | |
| K1232 | Ubiquitination | Uniprot | |
| T1238 | Phosphorylation | Uniprot | |
| K1248 | Ubiquitination | Uniprot | |
| K1272 | Ubiquitination | Uniprot | |
| K1280 | Ubiquitination | Uniprot | |
| K1290 | Ubiquitination | Uniprot | |
| S1312 | Phosphorylation | Uniprot |
研究背景
Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.
Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding.
Phosphorylated in response to DNA damage by ATM and/or ATR.
Nucleus.
Note: Observed in spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites. They are frequently interlinked through BLM-associated ultra-fine DNA bridges.
Interacts with FANCD2; the interaction is direct. Interacts with FANCL. Interacts with MTMR15/FAN1. Interacts with POLN.
The C-terminal 30 residues are probably required for function in DNA repair.
研究領域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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