製品: Phospho-HIPK2 (Tyr354) Antibody
カタログ: AF3538
タンパク質の説明: Rabbit polyclonal antibody to Phospho-HIPK2 (Tyr354)
アプリケーション: ELISA(peptide)
反応性: Human, Mouse, Rat
分子量: 140kD; 131kD(Calculated).
ユニプロット: Q9H2X6
RRID: AB_2846852

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製品説明

ソース:
Rabbit
アプリケーション:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-HIPK2 (Tyr354) Antibody detects endogenous levels of HIPK2 only when phosphorylated at Tyr354.
RRID:
AB_2846852
引用形式: Affinity Biosciences Cat# AF3538, RRID:AB_2846852.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

hHIPk 2; hHIPk2; HIPK 2; Hipk2; HIPK2_HUMAN; Homeodomain interacting protein kinase 2; Homeodomain-interacting protein kinase 2; Nbak1; Nuclear body-associated kinase 1; PRO0593; Sialophorin tail-associated nuclear serine/threonine-protein kinase; Stank;

免疫原

免疫原:

A synthesized peptide derived from human HIPK2 around the phosphorylation site of Tyr354.

Uniprot:
遺伝子(ID):
発現特異性:
Q9H2X6 HIPK2_HUMAN:

Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.

タンパク質配列:
MAPVYEGMASHVQVFSPHTLQSSAFCSVKKLKIEPSSNWDMTGYGSHSKVYSQSKNIPLSQPATTTVSTSLPVPNPSLPYEQTIVFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIENTSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQAPSSTSATISLANPEVSILNYPSTLYQPSAASMAAVAQRSMPLQTGTAQICARPDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGLLAQQAWPSGTQQILLPPAWQQLTGVATHTSVQHATVIPETMAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKSKQHQSSVRNVSTCEVSSSQAISSPQRSKRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTTRERQRQTIVIPDTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYSVQQRAGHNNANAFDTKGSLENHCTGNPRTIIVPPLKTQASEVLVECDSLVPVNTSHHSSSYKSKSSSNVTSTSGHSSGSSSGAITYRQQRPGPHFQQQQPLNLSQAQQHITTDRTGSHRRQQAYITPTMAQAPYSFPHNSPSHGTVHPHLAAAAAAAHLPTQPHLYTYTAPAALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQVPVSMGPRVLPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLSPAKVNQYPYI

PTMs - Q9H2X6 基板として

Site PTM Type Enzyme
Phosphorylation
K32 Sumoylation
K32 Ubiquitination
Y44 Phosphorylation
S46 Phosphorylation
T119 Phosphorylation
S121 Phosphorylation
T221 Phosphorylation
K228 Ubiquitination
K231 Ubiquitination
Y258 Phosphorylation
Y264 Phosphorylation
S336 Phosphorylation
Y340 Phosphorylation
K343 Ubiquitination
K355 Ubiquitination
S359 Phosphorylation
T360 Phosphorylation P00519 (ABL1)
Y361 Phosphorylation Q9H2X6 (HIPK2) , P12931 (SRC)
S364 Phosphorylation Q9H2X6 (HIPK2)
Y367 Phosphorylation
Y423 Phosphorylation
K430 Ubiquitination
Y443 Phosphorylation
K506 Ubiquitination
K514 Ubiquitination
K552 Ubiquitination
Y558 Phosphorylation
K565 Ubiquitination
T566 Phosphorylation
S668 Phosphorylation
K671 Ubiquitination
T796 Phosphorylation
K803 Ubiquitination
K805 Ubiquitination
S826 Phosphorylation
S827 Phosphorylation
T838 Phosphorylation
S848 Phosphorylation
T880 Phosphorylation Q9H2X6 (HIPK2)
S882 Phosphorylation Q9H2X6 (HIPK2)
S924 Phosphorylation
S934 Phosphorylation
K953 Sumoylation
T1114 Phosphorylation
Y1136 Phosphorylation
R1151 Methylation
S1188 Phosphorylation
K1191 Sumoylation
K1191 Ubiquitination
Y1197 Phosphorylation

PTMs - Q9H2X6 酵素として

Substrate Site Source
O00257 (CBX4) T497 Uniprot
O15297 (PPM1D) S85 Uniprot
O43255 (SIAH2) T26 Uniprot
O43255 (SIAH2) S28 Uniprot
O43255 (SIAH2) S68 Uniprot
P04637 (TP53) S46 Uniprot
P16220 (CREB1) S271 Uniprot
P17096 (HMGA1) S36 Uniprot
P17096-2 (HMGA1) T42 Uniprot
P17096 (HMGA1) T53 Uniprot
P17096-2 (HMGA1) T67 Uniprot
P17096 (HMGA1) T78 Uniprot
P17676 (CEBPB) T235 Uniprot
P18846 (ATF1) S198 Uniprot
P26367-1 (PAX6) T281 Uniprot
P26367-2 (PAX6) T295 Uniprot
P26367-1 (PAX6) T304 Uniprot
P26367-2 (PAX6) T317 Uniprot
P26367-1 (PAX6) T373 Uniprot
P26367-2 (PAX6) T387 Uniprot
P29590 (PML) S8 Uniprot
P29590 (PML) S36 Uniprot
P29590 (PML) S38 Uniprot
P35222 (CTNNB1) S33 Uniprot
P35222 (CTNNB1) S37 Uniprot
P46527 (CDKN1B) S10 Uniprot
P51608 (MECP2) S80 Uniprot
P52945 (PDX1) S268 Uniprot
P56545 (CTBP2) S428 Uniprot
P57682 (KLF3) S71 Uniprot
P57682 (KLF3) S78 Uniprot
P57682 (KLF3) S92 Uniprot
P57682 (KLF3) S101 Uniprot
P57682 (KLF3) S108 Uniprot
P57682 (KLF3) S111 Uniprot
P57682 (KLF3) S216 Uniprot
P57682 (KLF3) S224 Uniprot
P57682 (KLF3) S250 Uniprot
Q01196-1 (RUNX1) S249 Uniprot
Q01196 (RUNX1) T273 Uniprot
Q01196-1 (RUNX1) S276 Uniprot
Q13363 (CTBP1) S422 Uniprot
Q96EB6 (SIRT1) S27 Uniprot
Q96EB6 (SIRT1) S682 Uniprot
Q9H2X6 (HIPK2) Y361 Uniprot
Q9H2X6 (HIPK2) S364 Uniprot
Q9H2X6 (HIPK2) T880 Uniprot
Q9H2X6 (HIPK2) S882 Uniprot
Q9H3D4-2 (TP63) T397 Uniprot
Q9H3D4 (TP63) T491 Uniprot
Q9P1Z0 (ZBTB4) T795 Uniprot
Q9P1Z0 (ZBTB4) T797 Uniprot
Q9P1Z0 (ZBTB4) T983 Uniprot

研究背景

機能:

Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.

PTMs:

Autophosphorylation at Tyr-361 in the activation loop activates the kinase and promotes nuclear localization.

Sumoylated. When conjugated it is directed to nuclear speckles. Desumoylated by SENP1 (By similarity). Sumoylation on Lys-32 is promoted by the E3 SUMO-protein ligase CBX4.

Ubiquitinated by FBXO3, WSB1 and SIAH1, leading to rapid proteasome-dependent degradation. The degradation mediated by FBXO3, but not ubiquitination, is prevented in the presence of PML. The degradation mediated by WSB1 and SIAH1 is reversibly reduced upon DNA damage.

Cleaved at Asp-923 and Asp-984 by CASP6 in a p53/TP53-dependent manner. The cleaved form lacks the autoinhibitory C-terminal domain (AID), resulting in a hyperactive kinase, which potentiates p53/TP53 Ser-46 phosphorylation and subsequent activation of the cell death machinery.

細胞の位置付け:

Nucleus>PML body. Cytoplasm.
Note: Concentrated in PML/POD/ND10 nuclear bodies. Small amounts are cytoplasmic.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.

サブユニット構造:

Interacts with CREB1, SIAH1, WSB1, CBX4, TRADD, p53/TP53, TP73, TP63, CREBBP, DAXX, P53DINP1, SKI, SMAD1, SMAD2 and SMAD3, but not SMAD4. Interacts with ATF1, PML, RUNX1, EP300, NKX1-2, NKX2-5, SPN/CD43, UBE2I, HMGA1, CTBP1, AXIN1, NLK, MYB, POU4F1, POU4F2, POU4F3, UBE2I, UBL1 and ZBTB4. Probably part of a complex consisting of p53/TP53, HIPK2 and AXIN1. Interacts with SP100; positively regulates TP53-dependent transcription. Interacts with SUMO1P1/SUMO5.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.

研究領域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

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