Phospho-NIK (Thr559) Antibody - #AF3552
| 製品: | Phospho-NIK (Thr559) Antibody |
| カタログ: | AF3552 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-NIK (Thr559) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 104kD(Calculated). |
| ユニプロット: | Q99558 |
| RRID: | AB_2846866 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3552, RRID:AB_2846866.
折りたたみ／展開
;MAP3K14; FTDCR1B; HS; HsNIK; M3K14_HUMAN; Map3k14; Mitogen activated protein kinase kinase kinase 14; Mitogen-activated protein kinase kinase kinase 14; NF kappa beta inducing kinase; NF-kappa-beta-inducing kinase; NIK; Serine/threonine-protein kinase NIK;
免疫原
A synthesized peptide derived from human NIK around the phosphorylation site of Thr559.
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
- Q99558 M3K14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP
PTMs - Q99558 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| Ubiquitination | Uniprot | ||
| Y40 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| T559 | Phosphorylation | O15111 (CHUK) , P41279 (MAP3K8) | Uniprot |
| S643 | Phosphorylation | Uniprot | |
| K650 | Ubiquitination | Uniprot | |
| Y682 | Phosphorylation | Uniprot | |
| S814 | Phosphorylation | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| K882 | Ubiquitination | Uniprot |
PTMs - Q99558 酵素として
| Substrate | Site | Source |
|---|---|---|
| O14920-1 (IKBKB) | S177 | Uniprot |
| O14920-1 (IKBKB) | S181 | Uniprot |
| O15111 (CHUK) | S176 | Uniprot |
| O15111 (CHUK) | S180 | Uniprot |
| P25963 (NFKBIA) | S32 | Uniprot |
| P25963 (NFKBIA) | S36 | Uniprot |
| P61160 (ACTR2) | Y202 | Uniprot |
| P61160 (ACTR2) | T237 | Uniprot |
| P61160 (ACTR2) | T238 | Uniprot |
| Q00653-4 (NFKB2) | S865 | Uniprot |
| Q00653 (NFKB2) | S866 | Uniprot |
| Q00653-4 (NFKB2) | S869 | Uniprot |
| Q00653 (NFKB2) | S870 | Uniprot |
研究背景
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
Cytoplasm.
Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100 (By similarity). Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91. Interacts with NLRP12; this interaction promotes proteasomal degradation of MAP3K14.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Intestinal immune network for IgA production. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.