Phospho-CRTC3 (Ser162) Antibody - #AF3576
| 製品: | Phospho-CRTC3 (Ser162) Antibody |
| カタログ: | AF3576 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-CRTC3 (Ser162) |
| アプリケーション: | ELISA(peptide) |
| 反応性: | Human, Mouse, Rat |
| 分子量: | 67kD(Calculated). |
| ユニプロット: | Q6UUV7 |
| RRID: | AB_2846890 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3576, RRID:AB_2846890.
折りたたみ／展開
CREB regulated transcription coactivator 3; CREB-regulated transcription coactivator 3; CRTC 3; CRTC3; CRTC3_HUMAN; FLJ21868; TORC 3; TORC-3; TORC3; Transducer of CREB protein 3; Transducer of regulated cAMP response element binding protein (CREB) 3; Transducer of regulated cAMP response element binding protein 3; Transducer of regulated cAMP response element-binding protein 3; Transducer of regulated CREB protein 3;
免疫原
A synthesized peptide derived from human CRTC3 around the phosphorylation site of Ser162.
Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
- Q6UUV7 CRTC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAASPGSGSANPRKFSEKIALHTQRQAEETRAFEQLMTDLTLSRVQFQKLQQLRLTQYHGGSLPNVSQLRSSASEFQPSFHQADNVRGTRHHGLVERPSRNRFHPLHRRSGDKPGRQFDGSAFGANYSSQPLDESWPRQQPPWKDEKHPGFRLTSALNRTNSDSALHTSALSTKPQDPYGGGGQSAWPAPYMGFCDGENNGHGEVASFPGPLKEENLLNVPKPLPKQLWETKEIQSLSGRPRSCDVGGGNAFPHNGQNLGLSPFLGTLNTGGSLPDLTNLHYSTPLPASLDTTDHHFGSMSVGNSVNNIPAAMTHLGIRSSSGLQSSRSNPSIQATLNKTVLSSSLNNHPQTSVPNASALHPSLRLFSLSNPSLSTTNLSGPSRRRQPPVSPLTLSPGPEAHQGFSRQLSSTSPLAPYPTSQMVSSDRSQLSFLPTEAQAQVSPPPPYPAPQELTQPLLQQPRAPEAPAQQPQAASSLPQSDFQLLPAQGSSLTNFFPDVGFDQQSMRPGPAFPQQVPLVQQGSRELQDSFHLRPSPYSNCGSLPNTILPEDSSTSLFKDLNSALAGLPEVSLNVDTPFPLEEELQIEPLSLDGLNMLSDSSMGLLDPSVEETFRADRL
PTMs - Q6UUV7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K18 | Sumoylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| Y58 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S67 | Phosphorylation | Uniprot | |
| R87 | Methylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| K147 | Sumoylation | Uniprot | |
| T160 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | P57059 (SIK1) | Uniprot |
| K174 | Sumoylation | Uniprot | |
| K213 | Sumoylation | Uniprot | |
| K222 | Sumoylation | Uniprot | |
| K226 | Sumoylation | Uniprot | |
| K232 | Sumoylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S332 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | P57059 (SIK1) | Uniprot |
| S373 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S396 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T436 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| Y448 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| T577 | Phosphorylation | Uniprot |
研究背景
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Phosphorylation/dephosphorylation states of Ser-273 are required for regulating transduction of CREB activity (By similarity). CRTCs/TORCs are inactive when phosphorylated, and active when dephosphorylated at this site (By similarity). May be phosphorylated at Ser-391 by MAPK3/ERK1 and/or MAPK1/ERK2 or by some cyclin-dependent kinases such as CDK1,CDK2 or CDK5. Following adenylyl cyclase activation, dephosphorylated at Ser-162 and Ser-273 resulting in its dissociation from 14-3-3 proteins probably promoting CRTC3 translocation into the nucleus.
Nucleus. Cytoplasm.
Note: Appears to be mainly nuclear (PubMed:15454081). Translocates to the nucleus following adenylyl cyclase or MAP kinase activation (PubMed:30611118).
Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interacts (when phosphorylated at Ser-162 and Se-273) with 14-3-3 proteins. Interacts with YWHAE. Interacts (when phosphorylated at Ser-391) with phosphatase PP2A catalytic subunit PPP2CA and regulatory subunits PPP2R1A and PPP2R2A. Interacts, via the N-terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription.
(Microbial infection) Interacts with HTLV-1 protein Tax; this interaction enhances tax transcriptional activity.
Belongs to the TORC family.
研究領域
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
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