Phospho-DDR1 (Tyr180) Antibody - #AF3612
製品: | Phospho-DDR1 (Tyr180) Antibody |
カタログ: | AF3612 |
タンパク質の説明: | Rabbit polyclonal antibody to Phospho-DDR1 (Tyr180) |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
分子量: | 101kD(Calculated). |
ユニプロット: | Q08345 |
RRID: | AB_2846926 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3612, RRID:AB_2846926.
折りたたみ/展開
CAK; CD 167; CD167; CD167 antigen-like family member A; CD167a; Cell adhesion kinase; DDR 1; DDR; DDR1; DDR1_HUMAN; Discoidin domain receptor; Discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; Discoidin receptor tyrosine kinase isoform a; EDDR 1; EDDR1; Epithelial discoidin domain receptor 1; Epithelial discoidin domain-containing receptor 1; Epithelial specific receptor kinase; HGK2; Mammarian carcinoma kinase 10; Mammary carcinoma kinase 10; MCK-10; MCK10; NEP; Neuroepithelial tyrosine kinase; Neurotrophic tyrosine kinase receptor type 4; NTRK 4; NTRK4; OTTHUMP00000029343; OTTHUMP00000029344; OTTHUMP00000029345; OTTHUMP00000029346; OTTHUMP00000029347; OTTHUMP00000164863; OTTHUMP00000164867; OTTHUMP00000222080; Protein-tyrosine kinase 3A; Protein-tyrosine kinase RTK-6; PTK 3; PTK 3A protein tyrosine kinase 3A; PTK3; PTK3A; Receptor tyrosine kinase NEP; RTK 6; RTK6; TRK E; TRKE; Tyrosine kinase DDR; Tyrosine kinase receptor E; Tyrosine-protein kinase CAK;
免疫原
A synthesized peptide derived from human DDR1 around the phosphorylation site of Tyr180.
Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
- Q08345 DDR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPEALSSLLLLLLVASGDADMKGHFDPAKCRYALGMQDRTIPDSDISASSSWSDSTAARHSRLESSDGDGAWCPAGSVFPKEEEYLQVDLQRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMGWKDRWGQEVISGNEDPEGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMYLSEAVYLNDSTYDGHTVGGLQYGGLGQLADGVVGLDDFRKSQELRVWPGYDYVGWSNHSFSSGYVEMEFEFDRLRAFQAMQVHCNNMHTLGARLPGGVECRFRRGPAMAWEGEPMRHNLGGNLGDPRARAVSVPLGGRVARFLQCRFLFAGPWLLFSEISFISDVVNNSSPALGGTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTAILIGCLVAIILLLLLIIALMLWRLHWRRLLSKAERRVLEEELTVHLSVPGDTILINNRPGPREPPPYQEPRPRGNPPHSAPCVPNGSALLLSNPAYRLLLATYARPPRGPGPPTPAWAKPTNTQAYSGDYMEPEKPGAPLLPPPPQNSVPHYAEADIVTLQGVTGGNTYAVPALPPGAVGDGPPRVDFPRSRLRFKEKLGEGQFGEVHLCEVDSPQDLVSLDFPLNVRKGHPLLVAVKILRPDATKNARNDFLKEVKIMSRLKDPNIIRLLGVCVQDDPLCMITDYMENGDLNQFLSAHQLEDKAAEGAPGDGQAAQGPTISYPMLLHVAAQIASGMRYLATLNFVHRDLATRNCLVGENFTIKIADFGMSRNLYAGDYYRVQGRAVLPIRWMAWECILMGKFTTASDVWAFGVTLWEVLMLCRAQPFGQLTDEQVIENAGEFFRDQGRQVYLSRPPACPQGLYELMLRCWSRESEQRPPFSQLHRFLAEDALNTV
PTMs - Q08345 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S45 | Phosphorylation | Uniprot | |
K112 | Ubiquitination | Uniprot | |
K153 | Ubiquitination | Uniprot | |
N211 | N-Glycosylation | Uniprot | |
S213 | Phosphorylation | Uniprot | |
N260 | N-Glycosylation | Uniprot | |
K449 | Ubiquitination | Uniprot | |
T469 | Phosphorylation | Uniprot | |
Y484 | Phosphorylation | Q08345 (DDR1) | Uniprot |
S496 | Phosphorylation | Uniprot | |
Y513 | Phosphorylation | Q08345 (DDR1) | Uniprot |
T519 | Phosphorylation | Uniprot | |
Y520 | Phosphorylation | Q08345 (DDR1) | Uniprot |
K613 | Methylation | Uniprot | |
K615 | Methylation | Uniprot | |
S631 | Phosphorylation | Uniprot | |
K646 | Ubiquitination | Uniprot | |
K663 | Ubiquitination | Uniprot | |
K671 | Ubiquitination | Uniprot | |
K680 | Ubiquitination | Uniprot | |
T701 | Phosphorylation | Uniprot | |
Y703 | Phosphorylation | Uniprot | |
Y740 | Phosphorylation | Q08345 (DDR1) | Uniprot |
S788 | Phosphorylation | Q9HC98 (NEK6) | Uniprot |
Y792 | Phosphorylation | Q08345 (DDR1) | Uniprot |
Y796 | Phosphorylation | Q08345 (DDR1) | Uniprot |
Y797 | Phosphorylation | Q08345 (DDR1) | Uniprot |
T912 | Phosphorylation | Uniprot |
PTMs - Q08345 酵素として
研究背景
Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Autophosphorylated in response to fibrillar collagen binding.
Glycosylation of Asn-211, but apparently not of Asn-260 or Asn-394, prevents autophosphorylation from occurring in the absence of collagen.
Cell membrane>Single-pass type I membrane protein.
Cell membrane>Single-pass type I membrane protein.
Secreted.
Cell membrane>Single-pass type I membrane protein.
Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
Homodimer. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2.
The Gly/Pro-rich domains may be required for an unusual geometry of interaction with ligand or substrates.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
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